RASSF6 Polyklonaler Antikörper

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• KD/KO Validated

RASSF6 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 11921-1-AP

Synonyme

Ras association domain-containing protein 6

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Galerie der Validierungsdaten

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  WB analysis of HT-29 using 11921-1-AP

  HT-29 cells were subjected to SDS PAGE followed by western blot with 11921-1-AP (RASSF6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 11921-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 11921-1-AP (RASSF6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11921-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11921-1-AP (RASSF6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 11921-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11921-1-AP (RASSF6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11921-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11921-1-AP (RASSF6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 11921-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 11921-1-AP (RASSF6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11921-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11921-1-AP (RASSF6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 11921-1-AP

  IP result of anti-RASSF6 (IP:11921-1-AP, 4ug; Detection:11921-1-AP 1:500) with HepG2 cells lysate 2400ug.

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  IHC staining of human ovary tumor using 11921-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11921-1-AP (RASSF6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human prostate cancer using 11921-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11921-1-AP (RASSF6 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human prostate cancer using 11921-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11921-1-AP (RASSF6 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HeLa using 11921-1-AP

  Immunofluorescent analysis of Hela cells, using RASSF6 antibody 11921-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-29-Zellen, COLO 320-Zellen, HeLa-Zellen, HepG2-Zellen, MDA-MB-453s-Zellen, PC-3-Zellen, U-937-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 10 publications below
• IHC: See 7 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

11921-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA RASSF6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RASSF6 fusion protein Ag2565
• Vollständiger Name: Ras association (RalGDS/AF-6) domain family member 6
• Berechnetes Molekulargewicht: 369 aa, 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC058835
• Gene symbol: RASSF6
• Gene ID (NCBI): 166824
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human,mouse	WB,IHC	Oncotarget; RASSF6 downregulation promotes the epithelial-mesenchymal transition and predicts poor prognosis in colorectal cancer.; Authors - Rui Zhou
human	WB	Mol Cancer; The novel RASSF6 and RASSF10 candidate tumour suppressor genes are frequently epigenetically inactivated in childhood leukaemias.; Authors - Hesson Luke B LB
		Epigenetics; RASSF6 exhibits promoter hypermethylation in metastatic melanoma and inhibits invasion in melanoma cells.; Authors - Jessica Mezzanotte
human	WB,IHC	J Genet Genomics; RASSF6-TRIM16 axis promotes cell proliferation, migration and invasion in esophageal squamous cell carcinoma.; Authors - Leilei Zheng ; KD Validated
human	WB	J Cell Physiol; MiR-496 promotes migration and epithelial-mesenchymal transition by targeting RASSF6 in colorectal cancer.; Authors - Hua Wang
human	WB,IHC	Ann Surg Oncol; Decreased expression of RASSF6 is a novel independent prognostic marker of a worse outcome in gastric cancer patients after curative surgery.; Authors - Wen Yugang Y