RASIP1 Polyklonaler Antikörper

RASIP1 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26064-1-AP

Synonyme

RAIN, Ras interacting protein 1, RASIP1

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Galerie der Validierungsdaten

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  WB analysis of mouse lung using 26064-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 26064-1-AP (RASIP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 26064-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26064-1-AP (RASIP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 26064-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26064-1-AP (RASIP1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 26064-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26064-1-AP (RASIP1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human lung using 26064-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 26064-1-AP (RASIP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 26064-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 26064-1-AP (RASIP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 26064-1-AP

  Immunofluorescent analysis of HepG2 cells using 26064-1-AP (RASIP1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Lungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

26064-1-AP bindet in WB, IHC, IF,ELISA RASIP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RASIP1 fusion protein Ag22036
• Vollständiger Name: Ras interacting protein 1
• Berechnetes Molekulargewicht: 963 aa, 103 kDa
• Beobachtetes Molekulargewicht: 115 kDa
• GenBank-Zugangsnummer: BC021860
• Gene symbol: RASIP1
• Gene ID (NCBI): 54922
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RASIP1, also named as Ras-interacting protein 1 or Rain, is a 963 amino acid protein, which contains one dilute domain and one Ras-associating domian. RASIP1 is highly expressed in heart and detected at lower levels in placenta and pancreas. RASIP1 is required for the proper formation of vascular structures that develop via both vasculogenesis and angiogenesis. RASIP1 acts as a critical and vascular-specific regulator of GTPase signaling, cell architecture, and adhesion, which is essential for endothelial cell morphogenesis and blood vessel tubulogenesis.