RARA Polyklonaler Antikörper

RARA Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 10331-1-AP

Synonyme

retinoic acid receptor, alpha, Retinoic acid receptor alpha, RAR-alpha, RAR alpha, RAR

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 10331-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10331-1-AP (RARA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10331-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10331-1-AP (RARA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10331-1-AP

  IP result of anti-RARA (IP:10331-1-AP, 4ug; Detection:10331-1-AP 1:2000) with HeLa cells lysate 1760 ug.

• 

  IHC staining of human breast cancer using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10331-1-AP (RARA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10331-1-AP (RARA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10331-1-AP (RARA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10331-1-AP (RARA Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10331-1-AP (RARA Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10331-1-AP (RARA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10331-1-AP (RARA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10331-1-AP (RARA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Hodengewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

10331-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA RARA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RARA fusion protein Ag0420
• Vollständiger Name: retinoic acid receptor, alpha
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 50-60 kDa
• GenBank-Zugangsnummer: BC008727
• Gene symbol: RARA
• Gene ID (NCBI): 5914
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Retinoic acid receptors (RARs) function as nuclear receptors that control gene expression in response to binding of the ligand retinoic acid (RA). Retinoic acid receptor alpha (RARα), also known as NR1B1 (nuclear receptor subfamily 1, group B, member 1), plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis. Formation of a complex with histone deacetylases might lead to inhibition of RARE DNA element binding and to transcriptional repression (PubMed:28167758). Transcriptional activation and RARE DNA element binding might be supported by the transcription factor KLF2 (PubMed:28167758).

Publikationen

Species	Application	Title
human	WB	Br J Cancer; All-trans retinoic acid enhances the cytotoxic effect of decitabine on myelodysplastic syndromes and acute myeloid leukaemia by activating the RARα-Nrf2 complex; Authors - Lu Wang
mouse	WB,IHC	Metabolism; Disruption of retinoid homeostasis induces RBP4 overproduction in diabetes: O-GlcNAcylation involved.; Authors - Shyi-Jang Shin
human	WB	Front Cell Dev Biol; Silybin Prevents Prostate Cancer by Inhibited the ALDH1A1 Expression in the Retinol Metabolism Pathway.; Authors - Ying Jiang
human	WB	Cell Signal; ATRA-mediated-crosstalk between stellate cells and Kupffer cells inhibits autophagy and promotes NLRP3 activation in acute liver injury.; Authors - Zhen Yu
human	IF	Andrology; A novel SNP in HUWE1 promoter confers increased risk of NOA by affecting the RA/RARα pathway in Chinese individuals; Authors - Xudong Shan
human	WB,CoIP	Biomedicines; Histone H3 Acetylation Is Involved in Retinoid Acid-Induced Neural Differentiation through Increasing Mitochondrial Function; Authors - Yang Zhang