RAP2A Polyklonaler Antikörper

RAP2A Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Rezensionen (1)

Kat-Nr. : 13789-1-AP

Synonyme

RbBP 30, RbBP-30, Ras-related protein Rap-2a, Ras related protein Rap 2a, RAP2

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Galerie der Validierungsdaten

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  WB analysis of A431 using 13789-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 13789-1-AP (RAP2A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13789-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13789-1-AP (RAP2A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13789-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13789-1-AP (RAP2A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13789-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13789-1-AP (RAP2A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 13789-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13789-1-AP (RAP2A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 13789-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13789-1-AP (RAP2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13789-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13789-1-AP (RAP2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MCF-7 using 13789-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 13789-1-AP (RAP2A antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, K-562-Zellen, Maushirngewebe, Mauslungengewebe, PC-3-Zellen
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:3000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

13789-1-AP bindet in WB, IHC, IF/ICC, ELISA RAP2A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAP2A fusion protein Ag4808
• Vollständiger Name: RAP2A, member of RAS oncogene family
• Berechnetes Molekulargewicht: 183 aa, 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC041333
• Gene symbol: RAP2A
• Gene ID (NCBI): 5911
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Sci Rep; miR-9 and miR-124 synergistically affect regulation of dendritic branching via the AKT/GSK3β pathway by targeting Rap2a.; Authors - Qian Xue
human	IHC, WB	Clin Exp Med; RAP2A promotes apoptosis resistance of hepatocellular carcinoma cells via the mTOR pathway.; Authors - Jing-Ru Yang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Joleen (Verified Customer) (05-29-2019)

RAP2A should be 21kD, no prominent bands appeared at that molecular weight.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

RAP2A Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:13789-1-AP)