RAP1GDS1 Polyklonaler Antikörper

RAP1GDS1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 10377-1-AP

Synonyme

Exchange factor smgGDS, GDS1, RAP1GDS1, SMG GDS protein, SmgGDS

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 10377-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10377-1-AP (RAP1GDS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10377-1-AP

  IP result of anti-RAP1GDS1 (IP:10377-1-AP, 3ug; Detection:10377-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human gliomas using 10377-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10377-1-AP (RAP1GDS1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 10377-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10377-1-AP (RAP1GDS1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

10377-1-AP bindet in WB, IP, IF, IHC, ELISA RAP1GDS1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Huhn, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAP1GDS1 fusion protein Ag0230
• Vollständiger Name: RAP1, GTP-GDP dissociation stimulator 1
• Berechnetes Molekulargewicht: 66 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: BC001851
• Gene symbol: RAP1GDS1
• Gene ID (NCBI): 5910
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GDS stimulates the GDP/GTP exchange reaction of a group of small GTP-binding proteins (G proteins), including at least c-Ki-ras p21, smg p21A, smg p21B, rhoA p21 and rhoB p21, by stimulating the dissociation of GDP from and the subsequent binding of GTP to each small G protein. The small GTPase Rap1, which is activated by a large variety of stimuli, functions in the control of integrin-mediated cell adhesion. RAP1GDS1 (synonym: GDS1)is one of RAP1 GTP-GDP dissociation stimulators.

Publikationen

Species	Application	Title
	IP	Nat Genet; Mutations in RABL3 alter KRAS prenylation and are associated with hereditary pancreatic cancer.; Authors - Sahar Nissim
chicken	WB,IF	Microbiol Spectr; Avian Hepatitis E Virus ORF2 Protein Interacts with Rap1b to Induce Cytoskeleton Rearrangement That Facilitates Virus Internalization.; Authors - Beibei Zhang
human	WB	Oncol Lett; SUMO4 small interfering RNA attenuates invasion and migration via the JAK2/STAT3 pathway in non-small cell lung cancer cells.; Authors - Jin-Xiao Liang
human,mouse	WB	Commun Biol; Vimar/RAP1GDS1 promotes acceleration of brain aging after flies and mice reach middle age; Authors - Ying Xiong