RAP1B Polyklonaler Antikörper

RAP1B Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 10840-1-AP

Synonyme

GTP binding protein smg p21B, K REV, RAL1B, RAP1B, Ras related protein Rap 1b

---

Galerie der Validierungsdaten

• 

  WB analysis using 10840-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10840-1-AP (RAP1B antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  WB analysis of A549 using 10840-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10840-1-AP (RAP1B antibody) at dilution of 1:300. .

• 

  WB analysis using 10840-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10840-1-AP (RAP1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10840-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10840-1-AP (RAP1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10840-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10840-1-AP (RAP1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10840-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10840-1-AP (RAP1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 10840-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 10840-1-AP (RAP1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human oesophagus cancer using 10840-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10840-1-AP (RAP1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 10840-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10840-1-AP (RAP1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, A549-Zellen, C6-Zellen, HepG2-Zellen, humanes Hirngewebe, Maushirngewebe, Mausnierengewebe, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Ösophaguskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

10840-1-AP bindet in WB, IP, IHC, IF, ELISA RAP1B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Huhn
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAP1B fusion protein Ag1282
• Vollständiger Name: RAP1B, member of RAS oncogene family
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC000176
• Gene symbol: RAP1B
• Gene ID (NCBI): 5908
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAP1B is a member of the RAS-like small GTP-binding protein superfamily. Members of this family regulate multiple cellular processes including cell adhesion and growth and differentiation. RAP1B localizes to cellular membranes and has been shown to regulate integrin-mediated cell signaling. It also plays a role in regulating outside-in signaling in platelets.

Publikationen

Species	Application	Title
human	WB	Mol Cell; Macrophage-Associated PGK1 Phosphorylation Promotes Aerobic Glycolysis and Tumorigenesis.; Authors - Yajuan Zhang
human	WB	Oncotarget; LncRNA MT1JP functions as a tumor suppressor by interacting with TIAR to modulate the p53 pathway.; Authors - Lihui Liu
human	WB	Int J Mol Sci; Molecular and Pharmacological Characterization of the Interaction between Human Geranylgeranyltransferase Type I and Ras-Related Protein Rap1B.; Authors - Sonja Hinz
chicken	WB,IP	Microbiol Spectr; Avian Hepatitis E Virus ORF2 Protein Interacts with Rap1b to Induce Cytoskeleton Rearrangement That Facilitates Virus Internalization.; Authors - Beibei Zhang
human	WB,IF	Viruses; ICP4-Associated Activation of Rap1b Facilitates Herpes Simplex Virus Type I (HSV-1) Infection in Human Corneal Epithelial Cells; Authors - Beibei Zhang
human	WB	Int J Biochem Cell Biol; Regulation of RAP1B by miR-139 suppresses human colorectal carcinoma cell proliferation.; Authors - Guo Haiyan H