RANKL Monoklonaler Antikörper

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• KD/KO Validated

RANKL Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

3F2E1

Kat-Nr. : 66610-1-Ig

Synonyme

CD254, hRANKL2, ODF, OPGL, OPTB2, Osteoprotegerin ligand, RANKL, sOdf, TNFSF11, TRANCE

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Galerie der Validierungsdaten

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  WB analysis using 66610-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66610-1-Ig (RANKL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66610-1-Ig

  WB result of RANKL antibody (66610-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RANKL transfected HeLa cells.

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  WB analysis of DC2.4 using 66610-1-Ig

  DC2.4 cells were subjected to SDS PAGE followed by western blot with 66610-1-Ig (RANKL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66610-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66610-1-Ig (RANKL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 66610-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 66610-1-Ig (RANKL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HUVEC using 66610-1-Ig

  HUVEC cells were subjected to SDS PAGE followed by western blot with 66610-1-Ig (RANKL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of NCCIT using 66610-1-Ig

  NCCIT cell were subjected to SDS PAGE followed by western blot with 66610-1-Ig (RANKL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 66610-1-Ig

  human spleen tissue were subjected to SDS PAGE followed by western blot with 66610-1-Ig (RANKL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 66610-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using RANKL antibody (66610-1-Ig, Clone: 3F2E1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, DC2.4-Zellen, HeLa-Zellen, humanes Milzgewebe, HUVEC-Zellen, JAR-Zellen, NCCIT-Zellen, RAW 264.7-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

66610-1-Ig bindet in WB, IHC, IF, ELISA RANKL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RANKL fusion protein Ag19975
• Vollständiger Name: tumor necrosis factor (ligand) superfamily, member 11
• Berechnetes Molekulargewicht: 317 aa, 35 kDa
• Beobachtetes Molekulargewicht: 35-38 kDa
• GenBank-Zugangsnummer: BC074890
• Gene symbol: TNFSF11
• Gene ID (NCBI): 8600
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TNFSF11 also known as RANKL, is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. RANKL is a polypeptide of 217 amino acids that exerts its biological activity both in a transmembrane form of about 40-45 kDa and in soluble one of 31 kDa (PMID: 15308315). The membrane-bound RANKL (mRANKL) is cleaved into a sRANKL by the metalloprotease-disintegrin TNF-alpha convertase (TACE) or a related metalloprotease (MP). RANKL induces osteoclast formation through its receptor, RANK, which transduces signals by recruiting adaptor molecules, such as the TNF receptor-associated factor (TRAF) family of proteins. RANKL was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. RANKL was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis.

Publikationen

Species	Application	Title
mouse	IHC	J Cancer; Gut microbiota deficiency ameliorates multiple myeloma and myeloma-related bone disease by Th17 cells in mice models; Authors - Jia Liu
human	WB, IF	Cell Death Dis; RANK promotes colorectal cancer migration and invasion by activating the Ca2+-calcineurin/NFATC1-ACP5 axis.; Authors - Qian Liang
mouse	IHC,IF,WB	Open Life Sci; Effect of periostin on bone metabolic and autophagy factors during tooth eruption in mice; Authors - Han Qin