RANGRF Polyklonaler Antikörper

RANGRF Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 10552-1-AP

Synonyme

RanGNRF, Ran-binding protein MOG1

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Galerie der Validierungsdaten

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  WB analysis of SGC-7901 using 10552-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 10552-1-AP (RANGRF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 10552-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10552-1-AP (RANGRF antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10552-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10552-1-AP (RANGRF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 10552-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10552-1-AP (RANGRF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 10552-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10552-1-AP (RANGRF Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10552-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10552-1-AP (RANGRF Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10552-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10552-1-AP (RANGRF Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 10552-1-AP

  Immunofluorescent analysis of HepG2 cells using 10552-1-AP (RANGRF antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SGC-7901-Zellen, HepG2-Zellen, Maushodengewebe, Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

10552-1-AP bindet in WB, IHC, IF/ICC, ELISA RANGRF und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RANGRF fusion protein Ag0824
• Vollständiger Name: RAN guanine nucleotide release factor
• Berechnetes Molekulargewicht: 18 kDa
• Beobachtetes Molekulargewicht: 18-22 kDa
• GenBank-Zugangsnummer: BC006486
• Gene symbol: RANGRF
• Gene ID (NCBI): 29098
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RANGRF, also named as MOG1, RANGNRF, HSPC165, HSPC236 and MDS5, regulates the intracellular trafficking of RAN. In cardiac cells it regulates the cell surface localization of SCN5A.

Publikationen

Species	Application	Title
mouse	IHC	Sci Transl Med; Gene therapy targeting protein trafficking regulator MOG1 in mouse models of Brugada syndrome, arrhythmias, and mild cardiomyopathy.; Authors - Gang Yu
		Stem Cell Res; Generation of a homozygous RANGRF knockout hiPSC line by CRISPR/Cas9 system; Authors - Meiling Jiang