RAD52 Monoklonaler Antikörper

RAD52 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

3H8G9

Kat-Nr. : 68283-1-Ig

Synonyme

RAD52, RAD52 homolog (S. cerevisiae)

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Galerie der Validierungsdaten

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  WB analysis using 68283-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68283-1-Ig (RAD52 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, 4T1-Zellen, HL-60-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68283-1-Ig bindet in WB, ELISA RAD52 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RAD52 fusion protein Ag27665
• Vollständiger Name: RAD52 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 46 aa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: NM_001297419
• Gene symbol: RAD52
• Gene ID (NCBI): 5893
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

In Saccharomyces cerevisiae, Rad52 functions as a critical mediator of homologous recombination (HR) by facilitating the loading of Rad51 onto ssDNA. However, in mammalian systems and other vertebrates, BRCA2 is known to function as the primary mediator of RAD51 filament formation, while prior studies have shown a limited role for RAD52 in HR.Additionally, loss of RAD52 has little consequence for cell viability. Recent evidence suggests that RAD52 may provide an alternative mediator pathway to BRCA2 function (PMID: 34928169). Several studies have suggested a link between the functional regulation of RAD52 and the protein modification by a small ubiquitin-like modifier (SUMO), sumoylation may play an important role in the nuclear transport of RAD52 (PMID:20190268). The calculated MW of Rad52 is 46 kDa, 68283-1-Ig can detect the band around 46 kDa.