RAC2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RAC2 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 10735-1-AP

Synonyme

EN 7, Gx, HSPC022, p21 Rac2, RAC2, Small G protein

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Galerie der Validierungsdaten

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  WB analysis using 10735-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10735-1-AP (RAC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HL-60 using 10735-1-AP

  IP result of anti-RAC2 (IP:10735-1-AP, 3ug; Detection:10735-1-AP 1:300) with HL-60 cells lysate 4000ug.

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  IHC staining of human tonsillitis using 10735-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10735-1-AP (RAC2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 10735-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10735-1-AP (RAC2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 10735-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10735-1-AP (RAC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 10735-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10735-1-AP (RAC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HL-60-Zellen, Mausmilzgewebe, Rattenmilzgewebe
• Erfolgreiche IP: HL-60-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Nierenzellkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below

Produktinformation

10735-1-AP bindet in WB, IP, IHC, ELISA RAC2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAC2 fusion protein Ag1202
• Vollständiger Name: ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2)
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC001485
• Gene symbol: RAC2
• Gene ID (NCBI): 5880
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAC2, also named as GX, belongs to the small GTPase superfamily and Rho family. RAC2 is a plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. RAC2 augments the production of reactive oxygen species (ROS) by NADPH oxidase. Defects in RAC2 are the cause of neutrophil immunodeficiency syndrome.

Publikationen

Species	Application	Title
mouse	WB	Nat Genet; In vivo RNAi screening identifies regulators of actin dynamics as key determinants of lymphoma progression.; Authors - Meacham Corbin E CE ; KD Validated
human	WB	J Exp Clin Cancer Res; Long noncoding RNA AFAP1-AS1 acts as a competing endogenous RNA of miR-423-5p to facilitate nasopharyngeal carcinoma metastasis through regulating the Rho/Rac pathway.; Authors - Yu Lian
human	WB	Front Mol Neurosci; Quantitative Proteomic Analysis of Mouse Sciatic Nerve Reveals Post-injury Upregulation of ADP-Dependent Glucokinase Promoting Macrophage Phagocytosis.; Authors - Kai Zhang
human	WB	Oncol Rep; Upregulation and hypomethylation of lncRNA AFAP1‑AS1 predicts a poor prognosis and promotes the migration and invasion of cervical cancer.; Authors - Hao Bo
human	WB	Biochem Biophys Res Commun; Hsa_circ_001659 serves as a novel diagnostic and prognostic biomarker for colorectal cancer.; Authors - Baoyu He
human	WB	Tumour Biol; AFAP1-AS1, a long noncoding RNA upregulated in lung cancer and promotes invasion and metastasis.; Authors - Zhaoyang Zeng