RABL2B Polyklonaler Antikörper

RABL2B Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 11588-1-AP

Synonyme

Rab like protein 2B, RABL2B

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Galerie der Validierungsdaten

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  WB analysis using 11588-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11588-1-AP (RABL2B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11588-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11588-1-AP (RABL2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 11588-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11588-1-AP (RABL2B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11588-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11588-1-AP (RABL2B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 11588-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11588-1-AP (RABL2B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of fetal human brain using 11588-1-AP

  IP result of anti-RABL2B (IP:11588-1-AP, 4ug; Detection:11588-1-AP 1:300) with fetal human brain tissue lysate 4000 ug.

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  IHC staining of mouse kidney using 11588-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11588-1-AP (RABL2B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 11588-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11588-1-AP (RABL2B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11588-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11588-1-AP (RABL2B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11588-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11588-1-AP (RABL2B antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: fetales humanes Hirngewebe, humanes Herzgewebe, humanes Hirngewebe, humanes Lebergewebe, humanes Nierengewebe, Maushirngewebe
• Erfolgreiche IP: fetales humanes Hirngewebe
• Erfolgreiche Detektion in IHC: Mausnierengewebe, humanes Lungenkarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

11588-1-AP bindet in WB, IP, IF, IHC, ELISA RABL2B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RABL2B fusion protein Ag2189
• Vollständiger Name: RAB, member of RAS oncogene family-like 2B
• Berechnetes Molekulargewicht: 229 aa, 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC024281
• Gene symbol: RABL2B
• Gene ID (NCBI): 11158
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IF	J Cell Sci; RABL2 positively controls localization of GPCRs in mammalian primary cilia.; Authors - Izumi Dateyama