RABGAP1L Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RABGAP1L Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 13894-1-AP

Synonyme

Rab GTPase-activating protein 1-like, isoform 10

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 13894-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13894-1-AP (RABGAP1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 13894-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13894-1-AP (RABGAP1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 13894-1-AP

  IP result of anti-RABGAP1L (IP:13894-1-AP, 4ug; Detection:13894-1-AP 1:1000) with HeLa cells lysate 3200ug.

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  IHC staining of human hepatocirrhosis using 13894-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 13894-1-AP (RABGAP1L antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 13894-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 13894-1-AP (RABGAP1L antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 13894-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 13894-1-AP (RABGAP1L antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 13894-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 13894-1-AP (RABGAP1L antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 13894-1-AP

  Immunofluorescent analysis of Hela cells, using RABGAP1L antibody 13894-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberzirrhosegewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

13894-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA RABGAP1L und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RABGAP1L fusion protein Ag4856
• Vollständiger Name: RAB GTPase activating protein 1-like
• Berechnetes Molekulargewicht: 815 aa, 93 kDa
• Beobachtetes Molekulargewicht: 93-105 kDa
• GenBank-Zugangsnummer: BC041888
• Gene symbol: RABGAP1L
• Gene ID (NCBI): 9910
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RABGAP1L, also known as TBC1D18, is a member of the Tre2/Bub2/Cdc16 (TBC)-domain-containing protein family. Overexpression of RABGAP1L potentiated the antiviral action of IFN against a panel of viruses, including IAV, and its function required a catalytically active TBC domain.

Publikationen

Species	Application	Title
human	IHC	Nat Commun; The oncogene AAMDC links PI3K-AKT-mTOR signaling with metabolic reprograming in estrogen receptor-positive breast cancer.; Authors - Emily Golden
human	WB,IF	Cell Rep; Restriction factor screening identifies RABGAP1L-mediated disruption of endocytosis as a host antiviral defense.; Authors - Sonja Fernbach
mouse	WB	J Cell Biol; TBC1D18 is a Rab5-GAP that coordinates endosome maturation together with Mon1; Authors - Shu Hiragi ; KD Validated