RABAC1 Polyklonaler Antikörper

RABAC1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 10542-1-AP

Synonyme

Prenylated Rab acceptor protein 1, PRAF1, PRA1 family protein 1, PRA1

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 10542-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10542-1-AP (RABAC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10542-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10542-1-AP (RABAC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10542-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10542-1-AP (RABAC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10542-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10542-1-AP (RABAC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human pancreas using 10542-1-AP

  human pancreas tissue were subjected to SDS PAGE followed by western blot with 10542-1-AP (RABAC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 10542-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10542-1-AP (RABAC1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas cancer using 10542-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10542-1-AP (RABAC1 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HEK-293 using 10542-1-AP

  Immunofluorescent analysis of HEK-293 cells using 10542-1-AP (RABAC1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HEK-293 using 10542-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 10542-1-AP (RABAC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, humanes Pankreasgewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

10542-1-AP bindet in WB, IHC, IF/ICC, ELISA RABAC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RABAC1 fusion protein Ag0830
• Vollständiger Name: Rab acceptor 1 (prenylated)
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC008950
• Gene symbol: RABAC1
• Gene ID (NCBI): 10567
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB,IHC	BMC Neurosci; A role for prenylated rab acceptor 1 in vertebrate photoreceptor development.; Authors - Dickison Virginia M VM
mouse,human	WB,IF	PLoS One; Di-arginine and FFAT-like motifs retain a subpopulation of PRA1 at ER-mitochondria membrane contact sites.; Authors - Ameair Abu Irqeba
mouse	WB	Biochim Biophys Acta Proteins Proteom; Phosphoproteomic response of cardiac endothelial cells to ischemia and ultrasound.; Authors - Uchenna Emechebe