RAB6A Polyklonaler Antikörper

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• KD/KO Validated

RAB6A Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Rezensionen (1)

Kat-Nr. : 10187-2-AP

Synonyme

Ras-related protein Rab-6A, Ras related protein Rab 6A, Rab-6, RAB6, Rab 6

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Galerie der Validierungsdaten

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  WB analysis using 10187-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10187-2-AP (RAB6A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10187-2-AP

  IP result of anti-RAB6A (IP:10187-2-AP, 4ug; Detection:10187-2-AP 1:1000) with mouse brain tissue lysate 1840 ug.

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  IF Staining of HeLa using 10187-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 10187-2-AP (RAB6A antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).F-actin is stained using CL555-phalloidin (red) and DNA is stained by DAPI (blue).

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  IF Staining of HeLa using 10187-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB6A antibody (10187-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 10187-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB6A antibody (10187-2-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Produktinformation

10187-2-AP bindet in WB, IF/ICC, IP, ELISA RAB6A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB6A fusion protein Ag0240
• Vollständiger Name: RAB6A, member RAS oncogene family
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC003617
• Gene symbol: RAB6A
• Gene ID (NCBI): 5870
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Members of the Rab subfamily of small GTPases play an important role in the regulation of intracellular transport routes. Rab6, localized to the Golgi apparatus, has been shown to be a regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER). The expression of Rab6 is ubiquitous.

Publikationen

Species	Application	Title
mouse	WB	Nat Struct Mol Biol; TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.; Authors - Xinxin Zuo
human	WB	J Clin Invest; A pro-tumorigenic secretory pathway activated by p53 deficiency in lung adenocarcinoma.; Authors - Xiaochao Tan
rat	IF	J Neuroimmunol; Autoantibody against the Rab6A/Rab6B in primary autoimmune cerebellar ataxia associated with Sjogren's syndrome: A case report.; Authors - Liyuan Guo
rat	WB	Neurosci Lett; Absence of motor impairments or pathological changes in TMEM230 knockout rats; Authors - Wenjuan Zhang
mouse	WB	Development; A crucial role for Arf6 in the response of commissural axons to Slit.; Authors - Mariko Kinoshita-Kawada ; KD Validated
human	WB	Nat Commun; SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors.; Authors - Seiichi Koike

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (01-25-2019)

Very good, both for WB and IF assays.By WB, it gives a strong specific band at the expected molecular weight; by IF, PFA fixation, it labels Rab6 at the Golgi and the cilia.

• Applications: Western Blot, Immunofluorescence,