RAB4A Polyklonaler Antikörper

RAB4A Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 10347-1-AP

Synonyme

Ras-related protein Rab-4A, Ras related protein Rab 4A, RAB4, HRES 1/RAB4, EC:3.6.5.2

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Galerie der Validierungsdaten

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  WB analysis using 10347-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10347-1-AP (RAB4A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10347-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10347-1-AP (RAB4A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 10347-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10347-1-AP (RAB4A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 10347-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10347-1-AP (RAB4A Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 10347-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10347-1-AP (RAB4A Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 10347-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB4A antibody (10347-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 10347-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB4A antibody (10347-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, Maushirngewebe, Neuro-2a-Zellen, PC-12-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 2 publications below

Produktinformation

10347-1-AP bindet in WB, IHC, IF/ICC, ELISA RAB4A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB4A fusion protein Ag0357
• Vollständiger Name: RAB4A, member RAS oncogene family
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 24-28 kDa
• GenBank-Zugangsnummer: BC002438
• Gene symbol: RAB4A
• Gene ID (NCBI): 5867
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The human RAB genes share structural and biochemical properties with the Ras gene superfamily, and RAB4 (synonym: RAB4A) is a small GTPase belonging to this Ras superfamily. Accumulating data suggests an important role for RAB proteins either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from endoplasmic reticulum to the Golgi complex and to secretory vesicles involves the movement of carrier vesicles, a process that appears to involve RAB protein function. RAB4 may also function as regulator along the receptor recycling pathway.

Publikationen

Species	Application	Title
		Nat Commun; CHML promotes liver cancer metastasis by facilitating Rab14 recycle.; Authors - Tian-Wei Chen
mouse	IF	MBio; Yersinia pestisTargets the Host Endosome Recycling Pathway during the Biogenesis of theYersinia-Containing Vacuole To Avoid Killing by Macrophages.; Authors - Michael G Connor
human	WB	Virology; Characterization of intracellular dynamics of inoculated PrP-res and newly generated PrP(Sc) during early stage prion infection in Neuro2a cells.; Authors - Takeshi Yamasaki
mouse	IF	Pathog Dis; Accumulation of EBI3 induced by virulent Mycobacterium tuberculosis inhibits apoptosis in murine macrophages.; Authors - Jia-Hui Deng
mouse	WB	FASEB J; Tsg101 positively regulates physiologic-like cardiac hypertrophy through FIP3-mediated endosomal recycling of IGF-1R.; Authors - Kobina Essandoh