RAB3IP Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RAB3IP Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Rezensionen (2)

Kat-Nr. : 12321-1-AP

Synonyme

RAB3IP/Rabin8, Rabin8, Rabin 3, Rab3A-interacting protein, Rab-3A-interacting protein

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Galerie der Validierungsdaten

• 

  WB analysis of human brain using 12321-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12321-1-AP (RAB3IP/Rabin8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12321-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12321-1-AP (RAB3IP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 12321-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12321-1-AP (RAB3IP/Rabin8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12321-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12321-1-AP (RAB3IP/Rabin8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 12321-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12321-1-AP (RAB3IP/Rabin8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 12321-1-AP

  IP result of anti-RAB3IP (IP:12321-1-AP, 3ug; Detection:12321-1-AP 1:1000) with mouse lung tissue lysate 4500ug.

• 

  IHC staining of human stomach cancer using 12321-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12321-1-AP (RAB3IP/Rabin8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 12321-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12321-1-AP (RAB3IP/Rabin8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 12321-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RAB3IP/Rabin8 antibody (12321-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Herzgewebe, humanes Hirngewebe, Mausherzgewebe, Mauslungengewebe, Rattenhirngewebe
• Erfolgreiche IP: Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 15 publications below
• IF: See 6 publications below
• IP: See 3 publications below

Produktinformation

12321-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA RAB3IP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hund, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB3IP fusion protein Ag2974
• Vollständiger Name: RAB3A interacting protein (rabin3)
• Berechnetes Molekulargewicht: 476 aa, 53 kDa
• Beobachtetes Molekulargewicht: 53/51/43 kDa
• GenBank-Zugangsnummer: BC015548
• Gene symbol: RAB3IP
• Gene ID (NCBI): 117177
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAB3IP, also hnown as Rabin8, is a deduced 476-amino acid protein containing a central coiled-coil domain, a bipartite nuclear localization signal, and a C-terminal endoplasmic reticulum retention motif. Rabin8 has several sites for N-glycosylation and phosphorylation. Rabin8 plays important roles in various cellular processes, including ciliogenesis.The GEF activity of Rabin8 towards Rab8 is essential for ciliogenesis and cyst apical membrane transport,but not for exocytosis of discoidal/fusiform-shaped vesicles. This antibody can recognize isoforms with predicted MW's of 53 kDa, 51 kDa, 43 kDa, 45 kDa, 33 kDa and 35 kDa, 29 kDa respectively.

Publikationen

Species	Application	Title
canine	WB,IF	Nat Cell Biol; A molecular network for de novo generation of the apical surface and lumen.; Authors - Bryant David M DM ; KD Validated
rat	WB, IF	Neuron; Chemical genetic identification of NDR1/2 kinase substrates AAK1 and Rabin8 Uncovers their roles in dendrite arborization and spine development.; Authors - Sila K Ultanir
mouse	WB, IF	PLoS Biol; GSK3β-Dzip1-Rab8 Cascade Regulates Ciliogenesis after Mitosis.; Authors - Boyan Zhang
human	WB	J Cell Biol; Chibby promotes ciliary vesicle formation and basal body docking during airway cell differentiation.; Authors - Michael C Burke
human	WB	EMBO J; Phosphoproteomic screening identifies Rab GTPases as novel downstream targets of PINK1.; Authors - Yu-Chiang Lai
human	WB	EMBO J; Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.; Authors - Tatsuo Miyamoto ; KO Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Silvia (Verified Customer) (09-30-2021)

Immunofluorescence (1:200) works well on NPC

• Applications: Immunofluorescence

FH

Sara (Verified Customer) (04-07-2020)

I got a quite similar staining to the one shown in the example figure of this product data sheet. Not very sharp staining (a bit all over the cell), but working. Next I will try in WB.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Spleen / B cells