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RAB39B Monoklonaler Antikörper

RAB39B Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2F4F7

Kat-Nr. : CL488-68014

Synonyme

RAB39B, Ras related protein Rab 39B

Galerie der Validierungsdaten

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  • Flow cytometry (FC) experiment of SH-SY5Y cells using CoraLite® Plus 488-conjugated RAB39B Monoclonal an (CL488-68014)

    FC experiment of SH-SY5Y using CL488-68014

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RAB39B (CL488-68014, Clone:2F4F7) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in FCSH-SY5Y-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-68014 bindet in FC (Intra) RAB39B und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

Getestete Reaktivität Hausschwein, human, Kaninchen, Maus, Ratte
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen RAB39B fusion protein Ag27170
Vollständiger Name RAB39B, member RAS oncogene family
Berechnetes Molekulargewicht 213 aa, 25 kDa
Beobachtetes Molekulargewicht25 kDa
GenBank-ZugangsnummerBC009714
Gene symbol RAB39B
Gene ID (NCBI) 116442
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAB39B is a member of the RAB GTPase family with a putative role in vesicle trafficking. RAB39B is predominantly expressed in neuronal cells and is localized to the endoplasmic reticulum, Golgi and trans‐Golgi recycling endosomes. Several reports suggested that RAB39B may regulate protein trafficking and the PI3K-AKT-mTOR pathway. Mutations in RAB39B are known to be associated with X-linked intellectual disability (XLID), Parkinson's disease, and autism.

Protokolle

Produktspezifische Protokolle
FC protocol for CL Plus 488 RAB39B antibody CL488-68014Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen