RAB2B Polyklonaler Antikörper

RAB2B Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 11756-1-AP

Synonyme

RAB2B, Ras related protein Rab 2B

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Galerie der Validierungsdaten

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  WB analysis of human brain using 11756-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11756-1-AP (RAB2B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 11756-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11756-1-AP (RAB2B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11756-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11756-1-AP (RAB2B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 11756-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11756-1-AP (RAB2B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11756-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11756-1-AP (RAB2B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11756-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11756-1-AP (RAB2B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, HeLa-Zellen, Maushirngewebe, Mausnierengewebe, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

11756-1-AP bindet in WB, IHC, ELISA RAB2B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB2B fusion protein Ag2352
• Vollständiger Name: RAB2B, member RAS oncogene family
• Berechnetes Molekulargewicht: 216 aa, 24 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC020839
• Gene symbol: RAB2B
• Gene ID (NCBI): 84932
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The protein belongs to the Rab family, members of which are small molecular weight guanosine triphosphatases (GTPases) that contain highly conserved domains involved in GTP binding and hydrolysis. The Rabs are membrane-bound proteins, involved in vesicular fusion and trafficking. This protein is a resident of pre-Golgi intermediates, and is required for protein transport from the endoplasmic reticulum (ER) to the Golgi complex. This antibody can recognize both RAB2A and RAB2B.

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; A dual role of ERGIC-localized Rabs in TMED10-mediated unconventional protein secretion; Authors - Yuxin Sun ; KD Validated
human	IHC	Dis Markers; Differential Plasma Proteins Identified via iTRAQ-Based Analysis Serve as Diagnostic Markers of Pancreatic Ductal Adenocarcinoma; Authors - Xiubing Chen
mouse	WB	Dev Cell; Setdb1 protects genome integrity in murine muscle stem cells to allow for regenerative myogenesis and inflammation; Authors - Pauline Garcia