RAB13 Polyklonaler Antikörper

RAB13 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11718-1-AP

Synonyme

GIG4, Ras-related protein Rab-13, Ras related protein Rab 13, Cell growth-inhibiting gene 4 protein, Cell growth inhibiting gene 4 protein

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Galerie der Validierungsdaten

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  WB analysis using 11718-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11718-1-AP (RAB13 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 11718-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11718-1-AP (RAB13 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  FC experiment of K-562 using 11718-1-AP

  1x10^6 K-562 cells were intracellularly stained with 0.4 ug RAB13 Polyclonal antibody (11718-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Maus-Kolongewebe, Mauslungengewebe, Ratten-Kolongewebe, Rattenlungengewebe
• Erfolgreiche Detektion in FC (Intra): K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

11718-1-AP bindet in WB, ELISA RAB13 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB13 fusion protein Ag2292
• Vollständiger Name: RAB13, member RAS oncogene family
• Berechnetes Molekulargewicht: 203 aa, 23 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC000799
• Gene symbol: RAB13
• Gene ID (NCBI): 5872
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAB13 (Ras-related protein Rab-13) is also named as GIG4. Rab13 is a member of the Rab GTPase family. RAB13 is highly expressed in malignant cells. Rab13 was highly expressed in BCSC of triple-negative breast cancer (TNBC) (PMID: 35395074). RAB13 is involved in a number of processes in LIHC, including MTORC1 signaling, MYC targets v1, G2M checkpoint, MITOTIC spindle, DNA repair, P53 pathway, glycolysis, PI3K-AKT-MTOR signaling, etc (PMID: 37803063). RAB13 enhances its circulation to the plasma membrane to promote breast cancer invasion. By influencing the tumor microenvironment, RAB13 maintains breast cancer stem cells (PMID: 37803063).