Proenkephalin-A Polyklonaler Antikörper

Proenkephalin-A Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 32272-1-AP

Synonyme

PENK, Proenkephalin A, Opioid growth factor, Met-enkephalin-Arg-Phe, Met-enkephalin-Arg-Gly-Leu

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  WB analysis using 32272-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32272-1-AP (PENK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 32272-1-AP

  Fetal human brain was subjected to SDS PAGE followed by western blot with 32272-1-AP (Proenkephalin-A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 32272-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32272-1-AP (Proenkephalin-A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 32272-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 32272-1-AP (Proenkephalin-A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 32272-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 32272-1-AP (Proenkephalin-A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 32272-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 32272-1-AP (Proenkephalin-A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 32272-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 32272-1-AP (Proenkephalin-A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 32272-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Proenkephalin-A antibody (32272-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: fetales humanes Hirngewebe, humanes Herzgewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

32272-1-AP bindet in WB, IHC, IF/ICC, ELISA Proenkephalin-A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Proenkephalin-A fusion protein Ag27972
• Vollständiger Name: proenkephalin
• Berechnetes Molekulargewicht: 267 aa, 31 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC032505
• Gene symbol: Proenkephalin A
• Gene ID (NCBI): 5179
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Proenkephalin-A (PENK) is also named as Synenkephalin, Met-enkephalin and Opioid growth factor (OGF), and belongs to the opioid neuropeptide precursor family. PENK is a neuropeptide that competes with and mimics the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress (PMID:7057924). PENK hypermethylation is also associated with bladder cancer, hepatocellular carcinoma, colorectal cancer, and prostate cancer (PMID:36403035). Proenkephalin (PENK) represents a new candidate to determine kidney function. This peptide is cleaved from the precursor peptide preproenkephalin A alongside enkephalins (endogenous opioids) and is filtrated in the glomerulus (PMID: 33636859).