Phospho-p70(S6K) (Thr389) Polyklonaler Antikörper

Phospho-p70(S6K) (Thr389) Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28735-1-AP

Synonyme

p70 alpha, p70 ribosomal S6 kinase alpha, p70 S6 kinase alpha, p70 S6K, p70 S6K 1, p70 S6K alpha, p70 S6KA, p70(S6K), p70(S6K) alpha, P70S6K1, Phospho-p70(S6K) (Thr389), PS6K, Ribosomal protein S6 kinase I, RPS6KB1, S6K, S6K beta 1, S6K1, STK14A



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMit IGF-1 behandelte MCF-7-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 16 publications below

Produktinformation

28735-1-AP bindet in WB, ELISA Phospho-p70(S6K) (Thr389) und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name ribosomal protein S6 kinase, 70kDa, polypeptide 1
Beobachtetes Molekulargewicht65-85 kDa
GenBank-ZugangsnummerNM_003161
Gene symbol RPS6KB1
Gene ID (NCBI) 6198
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Rps6kb1 gene encodes the 70 kDa ribosomal protein S6 kinase (p70S6K), which is a serine/threonine kinase regulated by phosphoinositide 3-kinase (PI3K)/mammalian target of rapamycin (mTOR) pathway. P70S6K plays a crucial role in controlling cell cycle, growth and survival. The PI3K/mTOR signalling pathway is one of the major mechanisms for controlling cell survival, proliferation and metabolism and is the central regulator of translation of some components of protein synthesis system. Due to alternative translation two isoform S6K1 proteins are known to exist in mammalian cells: p85 S6K1 and p70 S6K1, which is identical to p85 S6K but lacks its first 23 amino acids. In addition, mammalian cells express a second S6K1 isoform spanning 316 amino acids (p31 S6K1). mTOR is know to phosphorylate and thereby activate p70 S6K1 at T389 and p85 S6K1 at T412. (PMID: 25100792, PMID: 24970012, PMID: 21602892)

Protokolle

Produktspezifische Protokolle
WB protocol for Phospho-p70(S6K) (Thr389) antibody 28735-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Cell Death Differ

Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration

Authors - Huaizhen Liang
humanWB

Oxid Med Cell Longev

Alpha-Ketoglutarate Alleviates Neuronal Apoptosis Induced by Central Insulin Resistance through Inhibiting S6K1 Phosphorylation after Subarachnoid Hemorrhage

Authors - Peng-Fei Ding
humanWB

Mol Oncol

PMID: 29633504

Authors - Yi Xiao
humanWB

Aging (Albany NY)

UPF1 impacts on mTOR signaling pathway and autophagy in endometrioid endometrial carcinoma.

Authors - Minfen Zhang
humanWB

Int J Mol Sci

Effects of Cynaroside on Cell Proliferation, Apoptosis, Migration and Invasion though the MET/AKT/mTOR Axis in Gastric Cancer.

Authors - Juanli Ji
humanWB

Cell Signal

Autophagy activation attenuates the circadian clock oscillators in U2OS cells via the ATG5 pathway

Authors - Dengke Gao