Phospho-TSC2 (Thr1462) Polyklonaler Antikörper

Phospho-TSC2 (Thr1462) Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Rezensionen (1)

Kat-Nr. : 29000-1-AP

Synonyme

FLJ43106, LAM, Phospho-TSC2 (Thr1462), TSC2, TSC4, Tuberin, tuberous sclerosis 2, Tuberous sclerosis 2 protein

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  WB analysis using 29000-1-AP

  Non-treated PC-3 and PDGF (HZ-1215) treated PC-3 cells were subjected to SDS PAGE followed by western blot with 29000-1-AP (Phospho-TSC2 (Thr1462) antibody) at dilution of 1:1000 incubated at 37℃ for 1 hour.

• 

  WB analysis using 29000-1-AP

  Non-treated NIH/3T3 and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29000-1-AP (Phospho-TSC2 (Thr1462) antibody) at dilution of 1:1000 incubated at 37℃ for 1 hour.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mit PDGF behandelte PC-3-Zellen, Mit PDGF behandelte NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

29000-1-AP bindet in WB, ELISA Phospho-TSC2 (Thr1462) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: tuberous sclerosis 2
• Berechnetes Molekulargewicht: 1807 aa, 201 kDa
• Beobachtetes Molekulargewicht: 200 kDa
• GenBank-Zugangsnummer: BC150300
• Gene symbol: TSC2
• Gene ID (NCBI): 7249
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide, 50% glycerol, and 0.05% BSA
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TSC2, also named as Tuberin, plays an essential role in the cellular energy response pathway. TSC2 forms a physical and functional complex with TSC1 that can inhibit the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. It has been reported that TSC2 can be phosphorylated at Thr1462 by Akt/PKB involved in stimulating cell growth and is activated by insulin. (PMID: 14651849, 12172553)

Publikationen

Species	Application	Title
human	WB	Research (Wash D C); The FTO Mediated N6-Methyladenosine Modification of DDIT4 Regulation with Tumorigenesis and Metastasis in Prostate Cancer; Authors - Yue Zhao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Cali (Verified Customer) (01-21-2025)

It is okay to detect endogenous pT1462 TSC2 with the right molecular weight (about 200 kd), although some possible non-specific bands exist.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse brain lysate

Phospho-TSC2 (Thr1462) Antibody Western Blot validation (1:1000 dilution) in mouse brain lysate (Cat no:29000-1-AP)