Phospho-STAT5A (Tyr694) Rekombinanter Antikörper

Phospho-STAT5A (Tyr694) Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

2P21

Kat-Nr. : CL647-80115

Synonyme

MGF, Phospho-STAT5A (Tyr694), STAT5, STAT5A

---

Galerie der Validierungsdaten

• 

  FC experiment of TF-1 using CL647-80115

  1X10^6 TF-1 cells untreated (dashed lines) or treated with GM-CSF (red) were intracellularly stained with 0.06 ug CoraLite® Plus 647 Anti-Human Phospho-STAT5A (Tyr694) (CL647-80115, Clone:2P21), or 0.06 ug Control Antibody (blue, solid line). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

• 

  FC experiment of TF-1 using CL647-80115

  1X10^6 TF-1 cells untreated (dashed lines) or treated with GM-CSF (red) were intracellularly stained with 0.06 ug CoraLite® Plus 647 Anti-Human Phospho-STAT5A (Tyr694) (CL647-80115, Clone:2P21), or 0.06 ug Control Antibody (blue, solid line). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

• 

  FC experiment of TF-1 using CL647-80115

  1X10^6 TF-1 cells untreated (dashed line, low) or treated with GM-CSF (100 ng/mL at 37℃ for 30 minutes after serum- starvation overnight, red, positive) were intracellularly stained with 0.13 ug CoraLite® Plus 647 Anti-Human Phospho-STAT5A (Tyr694) (CL647-80115, Clone:2P21) (red), or 0.13 ug Control Antibody (blue, solid line). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: TF-1-Zellen, Mit GM-CSF behandelte TF-1-Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-80115 bindet in FC (Intra) Phospho-STAT5A (Tyr694) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: signal transducer and activator of transcription 5A
• Berechnetes Molekulargewicht: 794 aa, 92 kDa
• Beobachtetes Molekulargewicht: 90-95 kDa
• GenBank-Zugangsnummer: BC027036
• Gene symbol: STAT5A
• Gene ID (NCBI): 6776
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different GHs. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Activation of STAT5A in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of human STAT5A is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of STAT5A in cells.