Phospho-PKC Delta (Ser359) Polyklonaler Antikörper

Phospho-PKC Delta (Ser359) Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 29562-1-AP

Synonyme

MAY1, Phospho-PKC Delta (Ser359), PKCD, PRKCD, Protein kinase C delta type, protein kinase C, delta

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Galerie der Validierungsdaten

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  WB analysis using 29562-1-AP

  Non-treated Jurkat, phosphatase inhibitor treated and λ phosphatase treated Jurkat cells were subjected to SDS PAGE followed by western blot with 29562-1-AP (Phospho-PKC Delta (Ser359) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  IF Staining of A431 using 29562-1-AP

  Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated A431 cells using Phospho-PKC Delta (Ser359) antibody (29562-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit λ-Phosphatase behandelte Jurkat-Zellen
• Erfolgreiche Detektion in IF: λ phosphatase treated A431 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

29562-1-AP bindet in WB, IF, ELISA Phospho-PKC Delta (Ser359) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: protein kinase C, delta
• Berechnetes Molekulargewicht: 78 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC043350
• Gene symbol: PRKCD
• Gene ID (NCBI): 5580
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein kinase C (PKC) was initially identified and characterized as a protein hydrolysis-activated kinase called protein kinase M. It has been established that PKC is a family of at least 12 serine/threonine kinases that is divided into three subfamilies: The classical PKCs (α, β1, β2, and γ), which are activated by diacylglycerol (DAG) and calcium; the novel PKCs (δ, ε, η, and θ) which are activated by DAG; and the atypical PKCs (ζ and λ/ι), which respond to neither DAG nor calcium. PKCδ, unlike other members of the PKC family, is unique in its regulation by tyrosine phosphorylation on multiple sites that determine activation, localization, and substrate specificity. PKCδ is activated by inflammatory mediators involved in the inflammatory response including lipopolysaccharide (LPS), tumor necrosis factor (TNF) and interleukin-1 (IL-1). PKCδ activation requires multi-phosphorylation steps which triggers translocation from the cell cytosol to different subcellular compartments. (PMID: 30095599, PMID: 31323909, PMID: 30917487)

Publikationen

Species	Application	Title
mouse,rat	WB	Cancers (Basel); Tumor-Associated Fibroblast-Derived Exosomal circDennd1b Promotes Pituitary Adenoma Progression by Modulating the miR-145-5p/ONECUT2 Axis and Activating the MAPK Pathway; Authors - Qian Jiang