Phospho-PDH E1 Alpha (Ser300) Rekombinanter Antikörper

Phospho-PDH E1 Alpha (Ser300) Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

2E19

Kat-Nr. : 80572-1-RR

Synonyme

p PDH E1 Alpha, p PDH E1 Alpha (Ser300), p PDH E1 Alpha Ser300, PDH E1 Alpha (Ser300), Phospho PDH E1 Alpha

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  WB analysis using 80572-1-RR

  Non-treated and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 80572-1-RR (Phospho-PDH E1 Alpha (Ser300) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control.

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  WB analysis of HSC-T6 using 80572-1-RR

  Non-treated and Calyculin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 80572-1-RR (Phospho-PDH E1 Alpha (Ser300) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control.

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  WB analysis of NIH/3T3 using 80572-1-RR

  Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80572-1-RR (Phospho-PDH E1 Alpha (Ser300) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control.

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  IF Staining of HepG2 using 80572-1-RR

  Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated HepG2 cells using Phospho-PDH E1 Alpha (Ser300) antibody (80572-1-RR, Clone: 2E19 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Mit Calyculin A behandelte HEK-293-Zellen, Mit Calyculin A behandelte HeLa-Zellen, mit Calyculin A behandelte HSC-T6-Zellen, mit Calyculin A behandelte NIH/3T3-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IF/ICC: λ phosphatase treated HepG2 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IP: See 1 publications below

Produktinformation

80572-1-RR bindet in WB, IF/ICC, IP, ELISA Phospho-PDH E1 Alpha (Ser300) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: pyruvate dehydrogenase (lipoamide) alpha 1
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC002406
• Gene symbol: PDHA1
• Gene ID (NCBI): 5160
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDH E1 Alpha (PDHA1), as the major component of PDH, can be phosphorylated and inactivated by PDHK1. PDHA1 contains three serine residues (Ser232, Ser293, or Ser300) that can be reversibly phosphorylated by a dedicated family of four inhibitory pyruvate dehydrogenase kinases (PDHK1-4) and two reactivating phosphatases (PDP1, 2). Hypoxia induces the expression of PDHK1 and PDHK3 and hyperphosphorylates PDHA1. Phosphorylated PDHA1 inactivates the whole pyruvate dehydrogenase complex (PDC), reduces pyruvate entering into the tricarboxylic acid (TCA) cycle for oxidative phosphorylation, enhances the Warburg effect and promotes tumorigenesis. (PMID: 30993888, PMID: 34749809)

Publikationen

Species	Application	Title
	WB,IP	Science; Boosting neuronal activity-driven mitochondrial DNA transcription improves cognition in aged mice; Authors - Wenwen Li
human,mouse	WB	Nat Commun; NOTCH1 mitochondria localization during heart development promotes mitochondrial metabolism and the endothelial-to-mesenchymal transition in mice; Authors - Jie Wang
human	WB	Cancer Discov; Copper Drives Remodeling of Metabolic State and Progression of Clear Cell Renal Cell Carcinoma; Authors - Megan E Bischoff