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Phospho-P53 (Ser15) Monoklonaler Antikörper

Phospho-P53 (Ser15) Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

CoraLite®555 Fluorescent Dye

CloneNo.

1H6G1

Kat-Nr. : CL555-67826

Synonyme

Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, P53, Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53

Galerie der Validierungsdaten

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  • Flow cytometry (FC) experiment of HT-29 cells using CoraLite®555-conjugated Phospho-P53 (Ser15) Monocl (CL555-67826)

    FC experiment of HT-29 using CL555-67826

    1X10^6 HT-29 cells untreated (dashed line) or treated with UV (red) HT-29 cells were intracellularly stained with 0.13 ug CoraLite®555 Anti-Human Phospho-P53 (Ser15) (CL555-67826, Clone:1H6G1) (red), or 0.13 ug Control Antibody (Blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Geprüfte Anwendungen

Erfolgreiche Detektion in FCUV treated HT-29 cells

Empfohlene Verdünnung

AnwendungVerdünnung
Durchflusszytometrie (FC)FC : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL555-67826 bindet in FC (Intra) Phospho-P53 (Ser15) und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name tumor protein p53
Berechnetes Molekulargewicht 44 kDa
Beobachtetes Molekulargewicht53 kDa
GenBank-ZugangsnummerBC003596
Gene symbol TP53
Gene ID (NCBI) 7157
Konjugation CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570 nm
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TP53, also known as P53 and NY-CO-13, belongs to the p53 family and has 9 isoforms. In SDS-Page, the observed molecular weight is about 53 kDa. TP53 acts as a tumor suppressor in many tumor types, including growth arrest or apoptosis depending on the physiological circumstances and cell types. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. TP53 Localizes in the nucleus in most cells but found in the cytoplasm in some cells. (PMID: 26166714; PMID: 25225161)