Phospho-INPPL1 (Tyr986/987) Polyklonaler Antikörper

Phospho-INPPL1 (Tyr986/987) Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29574-1-AP

Synonyme

INPPL 1, INPPL1, Phospho-INPPL1 (Tyr986/987), Protein 51C, SHIP 2, SHIP2

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Galerie der Validierungsdaten

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  WB analysis using 29574-1-AP

  Non-treated and UV treated HeLa cells were subjected to SDS PAGE followed by western blot with 29574-1-AP (Phospho-INPPL1 (Tyr986/987) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with INPPL1 antibody (26678-1-AP) subsequently.

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  WB analysis using 29574-1-AP

  Non-treated and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29574-1-AP (Phospho-INPPL1 (Tyr986/987) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with INPPL1 antibody (26678-1-AP) subsequently.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: UV treated HeLa cells, HeLa-Zellen, Mit UV behandelte NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

29574-1-AP bindet in WB, ELISA Phospho-INPPL1 (Tyr986/987) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: inositol polyphosphate phosphatase-like 1
• Berechnetes Molekulargewicht: 1258 aa, 139 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: BC140853
• Gene symbol: INPPL1
• Gene ID (NCBI): 3636
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Inositol polyphosphate phosphatase-like protein 1(INPPL1) belongs to the inositol and phosphatidylinositol 5-phosphatase family, also known as SH2 domain-containing inositol 5'-phosphatase 2(SHIP2), is ubiquitous in all organizations. SHIP2 is constitutively tyrosine-phosphorylated in chronic myeloid leukemia progenitor cells. In B cells, SHIP2 was most tyrosine phosphorylated after BCR and FC-γriib crosslinking and was associated with Shc, but not after BCR stimulation alone. It has also been shown that SHIP2 can be phosphorylated at Tyr-986 in COS-7 cells and Chinese hamster ovary cells overexpressing the insulin receptor. (PMID: 11349134)