PYGO2 Polyklonaler Antikörper

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• KD/KO Validated

PYGO2 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 11555-1-AP

Synonyme

1190004M21Rik, PYGO2, Pygopus homolog 2, pygopus homolog 2 (Drosophila)

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Galerie der Validierungsdaten

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  WB analysis of MDA-MB-453s using 11555-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 11555-1-AP (PYGO2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11555-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11555-1-AP (PYGO2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11555-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11555-1-AP (PYGO2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 11555-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 11555-1-AP (PYGO2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 11555-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 11555-1-AP (PYGO2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 11555-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 11555-1-AP (PYGO2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MDA-MB-453s-Zellen, C6-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

11555-1-AP bindet in WB, IHC, ELISA PYGO2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PYGO2 fusion protein Ag2111
• Vollständiger Name: pygopus homolog 2 (Drosophila)
• Berechnetes Molekulargewicht: 406 aa, 41 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC032099
• Gene symbol: PYGO2
• Gene ID (NCBI): 90780
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PYGO2, also named as Pygopus homolog 2, is a 406 animo acid protein, which contains 1 PHD-type zinc finger. PYGO2 is involved in signal transduction through the Wnt pathway and Binds to BCL9 via the PHD-type zinc finger motif, and thereby becomes part of the nuclear beta-catenin/TCF complex. PYGO2 is in a high proportion of breast and epithelial ovarian malignant tumors and is required for the growth of several cell lines derived from these carcinomas.

Publikationen

Species	Application	Title
human	WB,IHC	J Cancer; Pygo2 as a novel biomarker in gastric cancer for monitoring drug resistance by upregulating MDR1.; Authors - Dongdong Zhang ; KD Validated
human	WB	Nat Commun; Structural basis of the interaction between BCL9-Pygo and LDB-SSBP complexes in assembling the Wnt enhanceosome; Authors - Hongyang Wang