PYGL Polyklonaler Antikörper

PYGL Polyklonal Antikörper für IF, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55429-1-AP

Synonyme

GSD6, phosphorylase, glycogen, liver, PYGL

---

Galerie der Validierungsdaten

• 

  WB analysis of HepG2 using 55429-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55429-1-AP (PYGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55429-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55429-1-AP (PYGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 55429-1-AP

  IP Result of anti-PYGL (IP:55429-1-AP, 4ug; Detection:55429-1-AP 1:500) with HepG2 cells lysate 2400ug.

• 

  IHC staining of human skeletal muscle using 55429-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55429-1-AP (PYGL antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 55429-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55429-1-AP (PYGL antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 55429-1-AP

  Immunofluorescent analysis of HepG2 cells using 55429-1-AP (PYGL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, PC-3-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

55429-1-AP bindet in WB, IP, IF,ELISA PYGL und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: phosphorylase, glycogen, liver
• Berechnetes Molekulargewicht: 97 kDa
• Beobachtetes Molekulargewicht: 97-105 kDa
• GenBank-Zugangsnummer: NM_002863
• Gene symbol: PYGL
• Gene ID (NCBI): 5836
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PYGL belongs to the glycogen phosphorylase family. Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Defects in PYGL are the cause of glycogen storage disease type 6 (GSD6). This antibody is specific to PYGL. It has no cross reaction to PYGM and PYGB.