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PYCR1 Polyklonaler Antikörper

PYCR1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13108-1-AP

Synonyme

PIG45, P5CR 1, P5CR, P5C reductase 1, P5C



Geprüfte Anwendungen

Erfolgreiche Detektion in WBCOLO 320-Zellen, HeLa-Zellen, HT-1080.Zellen, humanes Hirngewebe, Mausembryogewebe, Maushirngewebe, Mauslebergewebe, NIH/3T3-Zellen, Rattenhirngewebe
Erfolgreiche IPMaushirngewebe
Erfolgreiche Detektion in IHChumanes Prostatakarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHepG2-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:4000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:100-1:400
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

13108-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA PYCR1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Zebrafisch
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen PYCR1 fusion protein Ag3764
Vollständiger Name pyrroline-5-carboxylate reductase 1
Berechnetes Molekulargewicht 319 aa, 33.8 kDa
Beobachtetes Molekulargewicht 33 kDa, 35 kDa
GenBank-ZugangsnummerBC022244
Gene symbol PYCR1
Gene ID (NCBI) 5831
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PYCR1,also named as P5CR1, belongs to the pyrroline-5-carboxylate reductase family. It is a housekeeping enzyme that catalyzes the last step in proline biosynthesis. PYCR1 can utilize both NAD and NADP, but has higher affinity for NAD. It is involved in the cellular response to oxidative stress. Mutation in PYCR1 will cause ARCL type II(ARCL2B ). Some mutation will cause DeBarsy syndrome (DBS) which is characterized by progeroid features, ophthalmological abnormalities, intrauterine growth retardation, and cutis laxa. The MW of PYCR1 is about 33-35 kDa. PYCR1 has 3 isoforms produced by alternative splicing. This antibody may have cross reaction to PYCR2 due to the high homology.

Protokolle

Produktspezifische Protokolle
WB protocol for PYCR1 antibody 13108-1-APProtokoll herunterladen
IHC protocol for PYCR1 antibody 13108-1-APProtokoll herunterladen
IF protocol for PYCR1 antibody 13108-1-APProtokoll herunterladen
IP protocol for PYCR1 antibody 13108-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB,IHC

Nature

Tumour-specific proline vulnerability uncovered by differential ribosome codon reading.

Authors - Fabricio Loayza-Puch
  • KO Validated
humanWB

Nature

Functional genomics reveal that the serine synthesis pathway is essential in breast cancer.

Authors - Possemato Richard R
zebrafishWB,IF

Nat Genet

Mutations in PYCR1 cause cutis laxa with progeroid features.

Authors - Reversade Bruno B
mouseWB

Cell Metab

Perturbed Redox Signaling Exacerbates a Mitochondrial Myopathy.

Authors - Sukru Anil Dogan
humanIHC

J Hepatol

Metabolic pathway analyses identify proline biosynthesis pathway as a promoter of liver tumorigenesis.

Authors - Zhaobing Ding
human,mouseWB,IF

Neuron

Loss of PYCR2 Causes Neurodegeneration by Increasing Cerebral Glycine Levels via SHMT2.

Authors - Nathalie Escande-Beillard

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Hyeonju (Verified Customer) (05-13-2024)

Too many non-specific bands are captured. ? I'm curious what the band is under pycr1 in lanes 6-9. Can you send me information about other proteins that react with the pycr1 antibody(if you have information about this)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse liver tissue
PYCR1 Antibody Western Blot validation (1:1000 dilution) in mouse liver tissue (Cat no:13108-1-AP)
FH

Daniel (Verified Customer) (02-05-2020)

The antibody stained the glomerulus of the mouse kidney.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Mouse kidney
PYCR1 Antibody Immunofluorescence, validation (1:200 dilution) in Mouse kidney (Cat no:13108-1-AP)