PTPN23 Polyklonaler Antikörper

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• KD/KO Validated

PTPN23 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 10472-1-AP

Synonyme

KIAA1471, His domain-containing protein tyrosine phosphatase, HD-PTP, HDPTP, HD PTP

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Galerie der Validierungsdaten

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  WB analysis of A375 using 10472-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 10472-1-AP (PTPN23 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10472-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10472-1-AP (PTPN23 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 10472-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 10472-1-AP (PTPN23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10472-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10472-1-AP (PTPN23 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10472-1-AP

  IP result of anti-PTPN23 (IP:10472-1-AP, 5ug; Detection:10472-1-AP 1:500) with HeLa cells lysate 1000ug.

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  IHC staining of human colon cancer using 10472-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10472-1-AP (PTPN23 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10472-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10472-1-AP (PTPN23 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10472-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10472-1-AP (PTPN23 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HepG2 using 10472-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PTPN23 antibody (10472-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 12 publications below
• IHC: See 2 publications below
• IF: See 6 publications below

Produktinformation

10472-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA PTPN23 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PTPN23 fusion protein Ag0765
• Vollständiger Name: protein tyrosine phosphatase, non-receptor type 23
• Berechnetes Molekulargewicht: 179 kDa
• Beobachtetes Molekulargewicht: 179 kDa
• GenBank-Zugangsnummer: BC004881
• Gene symbol: PTPN23
• Gene ID (NCBI): 25930
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PTPN23(Tyrosine-protein phosphatase non-receptor type 23) is also named as HD-PTP,PTP-TD14 and KIAA1471.It encodes a 185-kDa protein which is expressed in human endothelial fromthe umbilical cord and in human Kaposi-spindle cells(PMID:16408268).PTPN23 is a non-transmembrane protein tyrosine phosphatase which contributes to the signal transduction pathways involved in the regulation of cell migration and invasion(PMID:22510412).

Publikationen

Species	Application	Title
human	IF	Nat Commun; Concerted ESCRT and clathrin recruitment waves define the timing and morphology of intraluminal vesicle formation.; Authors - Eva Maria Wenzel
human	WB,IHC	Genes Dev; Suppression of protein tyrosine phosphatase N23 predisposes to breast tumorigenesis via activation of FYN kinase.; Authors - Siwei Zhang ; KD Validated
human	IF	EMBO J; ESCRT-mediated lysosome repair precedes lysophagy and promotes cell survival.; Authors - Maja Radulovic
human	WB, IF	Curr Biol; UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting.; Authors - Stefani Flavia F
	WB	Curr Biol; Recruitment of UBPY and ESCRT exchange drive HD-PTP-dependent sorting of EGFR to the MVB.; Authors - Nazim Ali ; KD Validated
human	WB	Cell Rep; Autophagy-Independent Lysosomal Targeting Regulated by ULK1/2-FIP200 and ATG9.; Authors - Jonathan M Goodwin