PTPN22 Polyklonaler Antikörper

PTPN22 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 11783-1-AP

Synonyme

Lymphoid phosphatase, LYP, LYP1, LYP2, PEP, PTPN22, PTPN8

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 11783-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11783-1-AP (PTPN22 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 11783-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11783-1-AP (PTPN22 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 11783-1-AP

  IP result of anti-PTPN22 (IP:11783-1-AP, 4ug; Detection:11783-1-AP 1:300) with Jurkat cells lysate 3080ug.

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  IHC staining of human lymphoma using 11783-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11783-1-AP (PTPN22 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Daudi-Zellen, Jurkat-Zellen, Raji-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

11783-1-AP bindet in WB, IP, IF, IHC, ELISA PTPN22 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PTPN22 fusion protein Ag2397
• Vollständiger Name: protein tyrosine phosphatase, non-receptor type 22 (lymphoid)
• Berechnetes Molekulargewicht: 82 kDa, 92 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC017785
• Gene symbol: PTPN22
• Gene ID (NCBI): 26191
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PTPN22(Tyrosine-protein phosphatase non-receptor type 22) is also named as LyP,PEP,PTPN8.It belongs to the protein-tyrosine phosphatase family and non-receptor class 4 subfamily.It acts as negative regulator of T-cell receptor (TCR) signaling.Defects in PTPN22 are a cause of susceptibility to systemic lupus erythematosus (SLE). It has some isoforms produced by alternative splicing with calculated molecular mass of 79-92 kDa, and apparent molecular mass of 100-110 kDa.

Publikationen

Species	Application	Title
mouse	WB	J Exp Med; NLR Nod1 signaling promotes survival of BCR-engaged mature B cells through up-regulated Nod1 as a positive outcome.; Authors - Kyoko Hayakawa
mouse	WB，IP	Sci Signal; The adaptor protein TRAF3 inhibits interleukin-6 receptor signaling in B cells to limit plasma cell development.; Authors - Wai W Lin
human	WB	Sci Rep; TRAF3 enhances TCR signaling by regulating the inhibitors Csk and PTPN22.; Authors - Alicia M Wallis
rat	IF	Pflugers Arch; Anandamide produced by Ca(2+)-insensitive enzymes induces excitation in primary sensory neurons.; Authors - Varga Angelika A