PTP1B Polyklonaler Antikörper

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• KD/KO Validated

PTP1B Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

34

Kat-Nr. : 11334-1-AP

Synonyme

PTPN1, PTPN 1, PTP-1B, PTP 1B, Protein-tyrosine phosphatase 1B

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Galerie der Validierungsdaten

• 

  WB analysis of Sp2/0 using 11334-1-AP

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11334-1-AP

  WB result of PTP1B antibody (11334-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTP1B transfected Jurkat cells.

• 

  WB analysis of human testis using 11334-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 11334-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11334-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 11334-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11334-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 11334-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 11334-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11334-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11334-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11334-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 11334-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 11334-1-AP

  IP result of anti-PTP1B (IP:11334-1-AP, 4ug; Detection:11334-1-AP 1:500) with A431 cells lysate 2400ug.

• 

  IHC staining of human prostate cancer using 11334-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11334-1-AP (PTP1B antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of BxPC-3 using 11334-1-AP

  Immunofluorescent analysis of BxPC-3 cells using 11334-1-AP (PTP1B antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Sp2/0-Zellen, A431-Zellen, BxPC-3-Zellen, C6-Zellen, HeLa-Zellen, HL-60-Zellen, humanes Hodengewebe, humanes Plazenta-Gewebe, Jurkat-Zellen, Mauslebergewebe, Maus-Plazenta-Gewebe, NIH/3T3-Zellen, Rattenlebergewebe, THP-1-Zellen
• Erfolgreiche IP: A431-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: BxPC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 7 publications below
• WB: See 32 publications below
• IHC: See 8 publications below
• IF: See 6 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Produktinformation

11334-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA PTP1B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PTP1B fusion protein Ag1878
• Vollständiger Name: protein tyrosine phosphatase, non-receptor type 1
• Berechnetes Molekulargewicht: 435 aa, 50 kDa
• Beobachtetes Molekulargewicht: 45-55 kDa
• GenBank-Zugangsnummer: BC015660
• Gene symbol: PTP1B
• Gene ID (NCBI): 5770
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PTPN1 (protein tyrosine phosphatase, non-receptor type 1) is also named as PTP1B or PTP-1B, and belongs to the protein-tyrosine phosphatase family and non-receptor class 1 subfamily. PTPN1 is involved in the regulation of several important physiological pathways, which regulates both INS and leptin signaling, and interacts with the epidermal-and platelet-derived growth factor receptors (PMID: 20101100). The gene might harbor variants influencing susceptibility to INS resistance and type 2 diabetes (PMID:15919813). The molecular mass of PTPN1 is 50 kDa, but sometimes some truncated forms can be detected as 48, 46, 39, 37 kDa due to non-specific proteolytic cleavage of PTP1B in different cells (PMID: 18253097).

Publikationen

Species	Application	Title
rat	WB	J Ginseng Res; Ginsenoside compound K reduces ischemia/reperfusion-induced neuronal apoptosis by inhibiting PTP1B-mediated IRS1 tyrosine dephosphorylation; Authors - Jing Fu
human	WB	Aging (Albany NY); Glycochenodeoxycholic acid induces stemness and chemoresistance via the STAT3 signaling pathway in hepatocellular carcinoma cells.; Authors - Changying Shi
rat	WB	J Cell Physiol; Circular RNA circTET3 mediates migration of rat vascular smooth muscle cells by targeting miR-351-5p.; Authors - Qing-Ping Yao
mouse	WB,IHC	FASEB J; PMID: 32100381; Authors - Ping Yang
human	WB	Front Cell Dev Biol; Tubeimoside I Inhibits Cell Proliferation and Induces a Partly Disrupted and Cytoprotective Autophagy Through Rapidly Hyperactivation of MEK1/2-ERK1/2 Cascade via Promoting PTP1B in Melanoma.; Authors - Juan Du ; KD Validated
mouse	WB	Eur J Pharmacol; Oleanolic acid derivative alleviates cardiac fibrosis through inhibiting PTP1B activity and regulating AMPK/TGF-β/Smads pathway; Authors - An-Hui Wang