PTPMT1 Polyklonaler Antikörper

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• KD/KO Validated

PTPMT1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Rezensionen (1)

Kat-Nr. : 11493-1-AP

Synonyme

DUSP23, MOSP, PLIP, PNAS 129, PTEN like phosphatase, PTPMT1

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Galerie der Validierungsdaten

• 

  WB analysis of mouse pancreas using 11493-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11493-1-AP (PTPMT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat pancreas using 11493-1-AP

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 11493-1-AP (PTPMT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 11493-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11493-1-AP (PTPMT1 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Pankreasgewebe, Ratten-Pankreasgewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

11493-1-AP bindet in WB, IF, IHC, ELISA PTPMT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PTPMT1 fusion protein Ag2063
• Vollständiger Name: protein tyrosine phosphatase, mitochondrial 1
• Berechnetes Molekulargewicht: 201 aa, 23 kDa
• Beobachtetes Molekulargewicht: 21-23 kDa
• GenBank-Zugangsnummer: BC020242
• Gene symbol: PTPMT1
• Gene ID (NCBI): 114971
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein Tyrosine Phosphatase localized to the Mitochondrial 1 (PTPMT1) is a highly conserved, dual-specific phosphatase that resides on the matrix-facing side of the inner mitochondrial membrane (IMM). PTPMT1 is a mitochondrial phosphatase and a vital regulator of mitochondrial activities. Current work points to roles for PTPMT1 in the regulation of mitochondrial respiration, dynamics, and ultrastructure through the modulation of pyruvate importation, the tuning of TCA cycle activity, and the synthesis of cardiolipin. PTPMT1 is highly expressed in pancreatic b cells, whose mitochondria have the important function of coupling glucose metabolism to the secretion of insulin. (PMID: 16337125, PMID: 16061174, PMID: 33335799)

Publikationen

Species	Application	Title
human	WB	Cell Death Dis; 4EBP1 senses extracellular glucose deprivation and initiates cell death signaling in lung cancer; Authors - Yanan Wang
mouse	WB,IF	Mol Cell Biol; A critical role of mitochondrial phosphatase Ptpmt1 in embryogenesis reveals a mitochondrial metabolic stress-induced differentiation checkpoint in embryonic stem cells.; Authors - Shen Jinhua J
human	WB,IHC	Cell Rep; Genome-wide CRISPR-Cas9 knockout library screening identified PTPMT1 in cardiolipin synthesis is crucial to survival in hypoxia in liver cancer.; Authors - Macus Hao-Ran Bao ; KO Validated
human,mouse	WB	Am J Physiol Cell Physiol; PTPMT1 protect cardiomyocytes from necroptosis induced by γ-ray irradiation through alleviate mitochondria injury; Authors - Jing Yi
human	WB	Adv Sci (Weinh); Phosphorylated SHMT2 Regulates Oncogenesis Through m6 A Modification in Lung Adenocarcinoma; Authors - Tianyu Han

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tongbin (Verified Customer) (08-25-2020)

This antibody detects PTPMT1 in mouse heart samples.

• Applications: Western Blot
• Cell Tissue Type: Heart