PTGR1 Polyklonaler Antikörper

PTGR1 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 13374-1-AP

Synonyme

LTB4DH, PGR1, PRG 1, prostaglandin reductase 1, PTGR1, ZADH3

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Galerie der Validierungsdaten

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  WB analysis using 13374-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13374-1-AP (PTGR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13374-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13374-1-AP (PTGR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13374-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13374-1-AP (PTGR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human colon using 13374-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 13374-1-AP (PTGR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 13374-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13374-1-AP (PTGR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 13374-1-AP

  IP result of anti-PTGR1 (IP:13374-1-AP, 4ug; Detection:13374-1-AP 1:500) with HepG2 cells lysate 3200ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, humanes Kolongewebe, humanes Lebergewebe, Mauslebergewebe
• Erfolgreiche IP: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

13374-1-AP bindet in WB, IP, ELISA PTGR1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PTGR1 fusion protein Ag4196
• Vollständiger Name: prostaglandin reductase 1
• Berechnetes Molekulargewicht: 329 aa, 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC035228
• Gene symbol: PTGR1
• Gene ID (NCBI): 22949
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PTGR1(Prostaglandin reductase 1) is also named as LTB4DH(NADP-dependent leukotriene B4 12-hydroxydehydrogenase) and belongs to the NADP-dependent oxidoreductase L4BD family. It catalyses the conversion of leukotriene B4 into biologically less reactive 12-oxo-leukotriene B4, a key step for its inactivation and it is a key oxidation step in the process of metabolic inactivation of leukotriene B(4) in various non-leukocyte tissues(PMID:8576264).

Publikationen

Species	Application	Title
human	WB	J Biol Chem; Modulation of Nitro-Fatty Acid Signaling: Prostaglandin Reductase-1 is a Nitroalkene Reductase.; Authors - Vitturi Dario A DA
human	WB	Mol Cancer Ther; Conditional Dependency of LP-184 on Prostaglandin Reductase 1 is Synthetic Lethal in Pancreatic Cancers with DNA Damage Repair Deficiencies; Authors - Diana Restifo