PTGES2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PTGES2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 10881-1-AP

Synonyme

mPGES 2, mPGE synthase-2, Membrane-associated prostaglandin E synthase-2, GBF1, EC:5.3.99.3

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 10881-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10881-1-AP (PTGES2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10881-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10881-1-AP (PTGES2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10881-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10881-1-AP (PTGES2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10881-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10881-1-AP (PTGES2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 10881-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 10881-1-AP (PTGES2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 10881-1-AP

  IP result of anti-PTGES2 (IP:10881-1-AP, 4ug; Detection:10881-1-AP 1:500) with mouse heart tissue lysate 4000ug.

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  IHC staining of human breast cancer using 10881-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10881-1-AP (PTGES2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 10881-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10881-1-AP (PTGES2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 10881-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10881-1-AP (PTGES2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 10881-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10881-1-AP (PTGES2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 10881-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10881-1-AP (PTGES2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 10881-1-AP

  Immunofluorescent analysis of Hela cells, using PTGES2 antibody 10881-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HepG2 using 10881-1-AP

  Immunofluorescent analysis of HepG2 cells using 10881-1-AP (PTGES2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, humanes Magengewebe, Mausherzgewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Herzgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 4 publications below

Produktinformation

10881-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA PTGES2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Caenorhabditis elegans, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PTGES2 fusion protein Ag1329
• Vollständiger Name: prostaglandin E synthase 2
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 30-35 kDa, 45 kDa
• GenBank-Zugangsnummer: BC011613
• Gene symbol: PTGES2
• Gene ID (NCBI): 80142
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PTGES2 (Prostaglandin E synthase 2) catalyzes the conversion of unstable intermediate of prostaglandin E2 H2 (PGH2) into the more stable prostaglandin E2 (PGE2) form. Its predicted size is 42 kDa, but it was detected one band of 35 kDa in the prostate and two bands of 30 kDa, 50 kDa in liver by western blot (PMID:17566096). PTGES2 protein is a homotetramer, whereas two PTGES2 molecules each form a strongly interacting dimer(PMID:19268562).

Publikationen

Species	Application	Title
human,mouse	WB	Brain Behav Immun; Elevated β-secretase 1 expression mediates CD4+ T cell dysfunction via PGE2 signalling in Alzheimer's disease.; Authors - Linbin Dai
rat	WB	Int Immunopharmacol; Curc-mPEG454, a PEGylated curcumin derivative, as a multi-target anti-fibrotic prodrug.; Authors - Shuang Xiao
human	WB,IHC	Tumour Biol; Prostaglandin E2 (PGE2) promotes proliferation and invasion by enhancing SUMO-1 activity via EP4 receptor in endometrial cancer.; Authors - Jieqi Ke ; KD Validated
rat	WB	J Neuroimmunol; Prostaglandin E2 increases the expression of cyclooxygenase-2 in cultured rat microglia.; Authors - Takayuki Nagano
Caenorhabditis elegans	WB	Int J Radiat Biol; Coupling of oxidative stress responses to tricarboxylic acid cycle and prostaglandin E2 alterations in Caenorhabditis elegans under extremely low-frequency electromagnetic field.; Authors - Yongyan Sun
human	IHC	Oncol Lett; Prostaglandin E2 triggers cytochrome P450 17α hydroxylase overexpression via signal transducer and activator of transcription 3 phosphorylation and promotes invasion in endometrial cancer.; Authors - Jieqi Ke