PTEN Polyklonaler Antikörper

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• KD/KO Validated

PTEN Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Kat-Nr. : 10047-1-AP

Synonyme

10q23del, BZS, MHAM, MMAC1, phosphatase and tensin homolog, PTEN, PTEN1, TEP1

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Galerie der Validierungsdaten

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  WB analysis using 10047-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10047-1-AP (PTEN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 10047-1-AP

  WB result of PTEN antibody (10047-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTEN transfected HEK-293T cells.

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  WB analysis of HeLa using 10047-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10047-1-AP (PTEN antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10047-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10047-1-AP (PTEN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293T using 10047-1-AP

  IP result of anti-PTEN (IP:10047-1-AP, 4ug; Detection:10047-1-AP 1:3000) with HEK-293T cells lysate 2040 ug.

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  IHC staining of human breast cancer using 10047-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10047-1-AP (PTEN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10047-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10047-1-AP (PTEN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, DU 145-Zellen, HEK-293T-Zellen, HeLa-Zellen
• Erfolgreiche IP: HEK-293T-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Produktinformation

10047-1-AP bindet in WB, IHC, IF, IP, ELISA PTEN und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PTEN fusion protein Ag0007
• Vollständiger Name: phosphatase and tensin homolog
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC005821
• Gene symbol: PTEN
• Gene ID (NCBI): 5728
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PTEN (also designated MMAC1), products of tumor suppressor genes, are found deleted in most human gliomas. The PTEN genes are also mutated in many other tumors, such as brain, breast, kidney and prostate cancers. PTEN is a protein tyrosine phosphatase that may terminate the signaling transduction pathways mediated by PI 3-kinase/Akt. PTEN has an apparent molecular weight of 55 kDa.

Publikationen

Species	Application	Title
human,mouse	WB,IHC,IF	Oxid Med Cell Longev; Knockdown of miR-214 Alleviates Renal Interstitial Fibrosis by Targeting the Regulation of the PTEN/PI3K/AKT Signalling Pathway; Authors - DongHua Hou
human	WB	Redox Biol; Impairment of Akt activity by CYP2E1 mediated oxidative stress is involved in chronic ethanol-induced fatty liver.; Authors - Tao Zeng
rat	WB,IHC	Cancers (Basel); Astragaloside IV Inhibits the Proliferation of Human Uterine Leiomyomas by Targeting IDO1; Authors - Tiantian Qiu
human	WB	Biol Direct; M2 macrophage-derived exosomes promote angiogenesis and improve cardiac function after myocardial infarction; Authors - Hongzhou Guo
human	WB	J Cell Mol Med; Knockdown of CENPK inhibits cell growth and facilitates apoptosis via PTEN-PI3K-AKT signalling pathway in gastric cancer.; Authors - Shusheng Wu
human,mouse	WB,IF	iScience; Smurf1 silencing restores PTEN expression that ameliorates progression of human glioblastoma and sensitizes tumor cells to mTORC1/C2 inhibitor Torin1.; Authors - Qin Xia ; KD Validated