PSPH Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PSPH Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

26

Rezensionen (1)

Kat-Nr. : 14513-1-AP

Synonyme

L 3 phosphoserine phosphatase, phosphoserine phosphatase, PSP, PSPase, PSPH

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Galerie der Validierungsdaten

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  WB analysis using 14513-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14513-1-AP (PSPH antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 14513-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 14513-1-AP (PSPH Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HL-60 using 14513-1-AP

  IP Result of anti-PSPH (IP:14513-1-AP, 4ug; Detection:14513-1-AP 1:300) with HL-60 cells lysate 3040ug.

• 

  IHC staining of human spleen using 14513-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14513-1-AP (PSPH Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 14513-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14513-1-AP (PSPH Antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 14513-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PSPH antibody (14513-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, HL-60-Zellen, MCF-7-Zellen, Rattenlebergewebe, U-87 MG-Zellen
• Erfolgreiche IP: HL-60-Zellen
• Erfolgreiche Detektion in IHC: humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 24 publications below
• IHC: See 7 publications below
• IP: See 1 publications below

Produktinformation

14513-1-AP bindet in WB, IP, IHC, IF, ELISA PSPH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSPH fusion protein Ag5972
• Vollständiger Name: phosphoserine phosphatase
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 25-28 kDa
• GenBank-Zugangsnummer: BC063614
• Gene symbol: PSPH
• Gene ID (NCBI): 5723
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSPH (phosphoserine phosphatase) is an enzyme, which is involved in the process of L-serine biosynthesis. PSPH mainly plays role in multiple aspects of cell behaviours such as proliferation and differentiation by producing precursors for the biosynthesis of diverse compounds including neurotransmitters, glycolipids and thymidine (PMID: 11237721, PMID: 19963421). Additionally, augmented PSPH level is correlated with the prognosis in multiple cancers including cutaneous squamous cell carcinoma (PMID: 21726982), breast cancer (PMID: 28931725), non-small cell lung cancer (PMID: 30662358), colorectal cancer (PMID: 24146633) and hepatocellular carcinoma (PMID: 25793315).

Publikationen

Species	Application	Title
		Science; mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle.; Authors - Issam Ben-Sahra
human	WB	Cell Metab; Epstein-Barr-Virus-Induced One-Carbon Metabolism Drives B Cell Transformation.; Authors - Liang Wei Wang
human	WB	Cell Metab; Serine metabolism antagonizes antiviral innate immunity by preventing ATP6V0d2-mediated YAP lysosomal degradation.; Authors - Long Shen
human	WB,IP	Nat Commun; PHGDH arginine methylation by PRMT1 promotes serine synthesis and represents a therapeutic vulnerability in hepatocellular carcinoma; Authors - Kui Wang
human	WB	Nat Commun; Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence; Authors - Yinsheng Wu
human	WB	Mol Cell; The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.; Authors - Youming Zhu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hua (Verified Customer) (11-30-2022)

Good antibody for WB.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HepG2 cells, mouse liver, and mouse brain

PSPH Antibody Western Blot validation (1:1000 dilution) in HepG2 cells, mouse liver, and mouse brain (Cat no:14513-1-AP)