PSME2 Polyklonaler Antikörper

PSME2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 12937-2-AP

Synonyme

REG-beta, REGbeta, REG beta, PA28beta, PA28B

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 12937-2-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12937-2-AP (PSME2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12937-2-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12937-2-AP (PSME2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12937-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12937-2-AP (PSME2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 12937-2-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12937-2-AP (PSME2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 12937-2-AP

  IP result of anti-PSME2 (IP:12937-2-AP, 4ug; Detection:12937-2-AP 1:500) with MCF-7 cells lysate 2000ug.

• 

  IHC staining of human kidney using 12937-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12937-2-AP (PSME2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 12937-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12937-2-AP (PSME2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 12937-2-AP

  Immunofluorescent analysis of MCF-7 cells using 12937-2-AP (PSME2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, humanes Hirngewebe, humanes Nierengewebe, Maus-Pankreasgewebe
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

12937-2-AP bindet in WB, IHC, IF/ICC, IP, ELISA PSME2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSME2 fusion protein Ag3986
• Vollständiger Name: proteasome (prosome, macropain) activator subunit 2 (PA28 beta)
• Berechnetes Molekulargewicht: 239 aa, 27 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC019885
• Gene symbol: PSME2
• Gene ID (NCBI): 5721
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The principal function of the proteasome is targeted degradation of intracellular proteins. Activity of the 20S proteasome is controlled by regulatory complexes that bind to the ends of the cylindrical proteasome. 11S regulator (REG or PA28), is a complex of 28 kDa subunits that is thought to activate proteasomes toward the production of antigenic peptides. Human PSME1 and PSME2 genes encode the two proteasome activators PA28 alpha and beta, respectively, which have been implicated in antigen processing for loading class I MHC molecules. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.

Publikationen

Species	Application	Title
	IF	PLoS Biol; Non-muscle myosins control radial glial basal endfeet to mediate interneuron organization; Authors - Brooke R D'Arcy
human	IHC	Front Genet; PSME2 identifies immune-hot tumors in breast cancer and associates with well therapeutic response to immunotherapy; Authors - Cen Wu
human,mouse	WB	Pharmacol Res; PD-L1 upregulation promotes drug-induced pulmonary fibrosis by inhibiting vimentin degradation; Authors - Qing Li