PSMD3 Polyklonaler Antikörper

PSMD3 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 12054-1-AP

Synonyme

P58, Proteasome subunit p58, PSMD3, RPN3, S3

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Galerie der Validierungsdaten

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  WB analysis using 12054-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12054-1-AP (PSMD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12054-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12054-1-AP (PSMD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 12054-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12054-1-AP (PSMD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12054-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12054-1-AP (PSMD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 12054-1-AP

  IP result of anti-PSMD3 (IP:12054-1-AP, 4ug; Detection:12054-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human colon cancer using 12054-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12054-1-AP (PSMD3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 12054-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 12054-1-AP (PSMD3 antibody) at dilution of 1:500 (under 10x lens).

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  IHC staining of human small intestine using 12054-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 12054-1-AP (PSMD3 antibody) at dilution of 1:500 (under 40x lens).

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  IHC staining of human breast cancer using 12054-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12054-1-AP (PSMD3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12054-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12054-1-AP (PSMD3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12054-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12054-1-AP (PSMD3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, humanes Hirngewebe, humanes Nierengewebe, Maushirngewebe, Mausnierengewebe, Rattennierengewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Dünndarmgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

12054-1-AP bindet in WB, IP, IF, IHC, ELISA PSMD3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSMD3 fusion protein Ag2684
• Vollständiger Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 3
• Berechnetes Molekulargewicht: 534 aa, 61 kDa
• Beobachtetes Molekulargewicht: 61 kDa
• GenBank-Zugangsnummer: BC012302
• Gene symbol: PSMD3
• Gene ID (NCBI): 5709
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSMD3, encoding proteasome (prosome, macropain) 26S subunit, non-ATPase 3, a member of the 26S proteasome family. PSMD3 is expressed in most of the tissues according to the RNA-Seq database. It has been reported that PSMD3 genotypes are associated with white blood cell (WBC) and neutrophil counts and have interactions with dietary fatty acids and carbohydrates on glucose-related traits. PSMD3 is located at ch17q21 and encodes a protein with a molecular weight of 61 kDa (PMID: 31013812).

Publikationen

Species	Application	Title
human	WB	Sci Rep; Insights into the ubiquitin-proteasome system of human embryonic stem cells.; Authors - Isabel Saez
human	WB	Protein Cell; DSSylation, a novel protein modification targets proteins induced by oxidative stress, and facilitates their degradation in cells.; Authors - Yinghao Zhang
human	WB	Cell Death Dis; ALG-2 couples T cell activation and apoptosis by regulating proteasome activity and influencing MCL1 stability.; Authors - Tian-Sheng He
human	WB,IF	Cell Mol Life Sci; The contribution of proteasomal impairment to autophagy activation by C9orf72 poly-GA aggregates; Authors - Mei Pu