PSMB6 Polyklonaler Antikörper

PSMB6 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 11684-2-AP

Synonyme

Y, Proteasome subunit beta type-6, LMPY, EC:3.4.25.1

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Galerie der Validierungsdaten

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  WB analysis using 11684-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11684-2-AP (PSMB6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11684-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11684-2-AP (PSMB6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11684-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11684-2-AP (PSMB6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11684-2-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11684-2-AP (PSMB6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 11684-2-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 11684-2-AP (PSMB6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Caco-2 using 11684-2-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 11684-2-AP (PSMB6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 11684-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11684-2-AP (PSMB6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11684-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11684-2-AP (PSMB6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 11684-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11684-2-AP (PSMB6 antibody) at dilution of 1:50 (under 25x lens).

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  IF Staining of HeLa using 11684-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11684-2-AP (PSMB6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Caco-2-Zellen, fetales humanes Hirngewebe, humanes Lebergewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

11684-2-AP bindet in WB, IHC, IF/ICC, ELISA PSMB6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSMB6 fusion protein Ag2296
• Vollständiger Name: proteasome (prosome, macropain) subunit, beta type, 6
• Berechnetes Molekulargewicht: 239 aa, 25 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC000835
• Gene symbol: PSMB6
• Gene ID (NCBI): 5694
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSMB6(Proteasome subunit beta type-6) is also named as LMPY, Y and belongs to the peptidase T1B family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.It may also catalyzes basal processing of intracellular antigens.It can be up-regulated in anaplastic thyroid cancer cell lines and down-regulated by IFNG/IFN-gamma (at protein level)(PMID:8066462;15613457).

Publikationen

Species	Application	Title
rat	WB	Cell Physiol Biochem; The Favored Mechanism for Coping with Acute Cold Stress: Upregulation of miR-210 in Rats.; Authors - Wenjin Guo
mouse	WB	J Biol Chem; Hepatocyte-specific Wtap deficiency promotes hepatocellular carcinoma by activating GRB2/ERK depending on downregulation of proteasome-related genes; Authors - Xinzhi Li
mouse	WB	Biochem Pharmacol; Melatonin inhibits angiotensin II-induced atrial fibrillation through preventing degradation of Ang II Type I Receptor-Associated Protein (ATRAP); Authors - Xin Xie
mouse	WB	JHEP Rep; Downregulation of hepatic METTL3 contributes to APAP-induced liver injury in mice; Authors - Chunhong Liu