PSMA/GCPII Monoklonaler Antikörper

PSMA/GCPII Monoklonal Antikörper für IF/ICC, IF-P

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte

Anwendung

IF/ICC, IF-P

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3G4E12

Kat-Nr. : CL488-66678

Synonyme

FOLH1, PSMA, FOLH, Folate hydrolase 1, FGCP

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  IF Staining of human prostate cancer using CL488-66678

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CoraLite® Plus 488 PSMA/GCPII antibody (CL488-66678, Clone: 3G4E12 ) at dilution of 1:200.

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  IF Staining of human prostate cancer using CL488-66678

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CoraLite® Plus 488 PSMA/GCPII antibody (CL488-66678, Clone: 3G4E12 ) at dilution of 1:200.

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  IF Staining of PC-3 using CL488-66678

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using CoraLite® Plus 488 PSMA/GCPII antibody (CL488-66678, Clone: 3G4E12 ) at dilution of 1:100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: humanes Prostatakarzinomgewebe, PC-3-Zellen
• Erfolgreiche Detektion in IF/ICC: PC-3-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66678 bindet in IF/ICC, IF-P PSMA/GCPII und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PSMA/GCPII fusion protein Ag16594
• Vollständiger Name: folate hydrolase (prostate-specific membrane antigen) 1
• Berechnetes Molekulargewicht: 719 aa, 81 kDa
• Beobachtetes Molekulargewicht: 100-120 kDa
• GenBank-Zugangsnummer: BC025672
• Gene symbol: PSMA
• Gene ID (NCBI): 2346
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSMA(Prostate-specific membrane antigen) is also named as FOLH1, FOLH, NAALAD1, PSM and belongs to the peptidase M28 family. PSMA is a 100-120 kDa integral transmembrane glycoprotein, considered to be a highly specific marker of the prostate gland, and has successfully been used as a marker of circulating prostatic epithelial cells(PMID:10074909; 15680901). It is involved in conversion of the major neurotransmitter (NAAG) to NAA and free glutamate. It has 8 isoforms produced by alternative splicing.