PSAT1 Polyklonaler Antikörper

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• KD/KO Validated

PSAT1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

65

Kat-Nr. : 10501-1-AP

Synonyme

PSAT, PSA, Phosphoserine aminotransferase, EPIP, EC:2.6.1.52

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Galerie der Validierungsdaten

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  WB analysis using 10501-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10501-1-AP (PSAT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10501-1-AP

  WB result of PSAT1 antibody (10501-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSAT1 transfected HEK-293 cells.

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  WB analysis of HEK-293 using 10501-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10501-1-AP (PSAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10501-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10501-1-AP (PSAT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10501-1-AP

  IP result of anti-PSAT1 (IP:10501-1-AP, 3ug; Detection:10501-1-AP 1:300) with HeLa cells lysate 2480ug.

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  IHC staining of human pancreas cancer using 10501-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10501-1-AP (PSAT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10501-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10501-1-AP (PSAT1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 10501-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10501-1-AP (PSAT1 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 10501-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PSAT1 antibody (10501-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 10501-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PSAT1 antibody (10501-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, Rattenhirngewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 8 publications below
• WB: See 63 publications below
• IHC: See 11 publications below
• IF: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

10501-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA PSAT1 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSAT1 fusion protein Ag0776
• Vollständiger Name: phosphoserine aminotransferase 1
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 37-40 kDa
• GenBank-Zugangsnummer: BC004863
• Gene symbol: PSAT1
• Gene ID (NCBI): 29968
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSAT1, also named PSA and PSAT, belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family and SerC subfamily. It catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine. PSAT1 represents a new interesting target for CRC therapy. (PMID:18221502) It may be implicated in altered serine metabolism and schizophrenia spectrum conditions. (PMID:20955740)

Publikationen

Species	Application	Title
human	WB	Nature; Stem cell function and stress response are controlled by protein synthesis.; Authors - Sandra Blanco
human	WB	Cell Metab; Serine metabolism antagonizes antiviral innate immunity by preventing ATP6V0d2-mediated YAP lysosomal degradation.; Authors - Long Shen
mouse	WB	Nat Immunol; PERK is a critical metabolic hub for immunosuppressive function in macrophages.; Authors - Lydia N Raines
mouse	WB	Cell Metab; PMID: 28111214; Authors - Eric H Ma
human	WB	Nat Commun; Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence; Authors - Yinsheng Wu
human	WB	Nat Commun; Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner.; Authors - Yun-Qian Wang

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