PSAP Polyklonaler Antikörper

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• KD/KO Validated

PSAP Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18397-1-AP

Synonyme

A1 activator, Cerebroside sulfate activator, Co beta glucosidase, Component C, CSAct

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  WB analysis using 18397-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18397-1-AP (PSAP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 18397-1-AP

  WB result of PSAP antibody (18397-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSAP transfected HepG2 cells.

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  WB analysis of L02 using 18397-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 18397-1-AP (PSAP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 18397-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18397-1-AP (PSAP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 18397-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18397-1-AP (PSAP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 18397-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18397-1-AP (PSAP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 18397-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18397-1-AP (PSAP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 18397-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 18397-1-AP (PSAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 18397-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 18397-1-AP (PSAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 18397-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 18397-1-AP (PSAP Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 18397-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 18397-1-AP (PSAP Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 18397-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 18397-1-AP (PSAP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 18397-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 18397-1-AP (PSAP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 18397-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 18397-1-AP (PSAP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 18397-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 18397-1-AP (PSAP antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A431 using 18397-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using PSAP antibody (18397-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen, L02-Zellen, MCF-7-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

18397-1-AP bindet in WB, IHC, IF/ICC, ELISA PSAP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSAP fusion protein Ag13264
• Vollständiger Name: prosaposin
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC001503
• Gene symbol: PSAP
• Gene ID (NCBI): 5660
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

proteins' (SAPs) that assist in the lysosomal hydrolysis of sphingolipids. After proteolytic processing of the presaposin protein, these 4 released polypeptides are functional activators. Saposin A was encoded by residues 60 to 143 of PSAP, saposin B by 195 to 275, saposin C by 311 to 390, and saposin D by 405 to 487. There are four 12-14 kDa heatstable glycoproteins. This antibody should recognize saposin B.