PRDX3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PRDX3 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

27

Rezensionen (1)

Kat-Nr. : 10664-1-AP

Synonyme

peroxiredoxin 3, MER5, EC:1.11.1.24, AOP-1, AOP1

---

Galerie der Validierungsdaten

• 

  WB analysis using 10664-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10664-1-AP (PRDX3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10664-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10664-1-AP (PRDX3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10664-1-AP

  WB result of PRDX3 antibody (10664-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX3 transfected HEK-293 cells.

• 

  WB analysis of MCF-7 using 10664-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10664-1-AP (PRDX3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa cells using 10664-1-AP

  WB result from Qing-Xi Yue, (PMID:20449493); “Control” and “GAD-treated” above the panel represent the control cells and HeLa cells treated with 10 μMGAD for 48 h, respectively. Each blot is the representative result of three independent experiments.

• 

  WB analysis of HeLa using 10664-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10664-1-AP (PRDX3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10664-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10664-1-AP (PRDX3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10664-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10664-1-AP (PRDX3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 10664-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10664-1-AP (PRDX3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10664-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10664-1-AP (PRDX3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10664-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRDX3 antibody (10664-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, LNCaP-Zellen, Mausherzgewebe, Maushirngewebe, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 23 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Produktinformation

10664-1-AP bindet in WB, IHC, IF/ICC, ELISA PRDX3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRDX3 fusion protein Ag1062
• Vollständiger Name: peroxiredoxin 3
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 26-28 kDa
• GenBank-Zugangsnummer: BC007062
• Gene symbol: PRDX3
• Gene ID (NCBI): 10935
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRDX3(Peroxiredoxin 3), also named as AOP1, HBC189 and MER5, belongs to the ahpC/TSA family. It is involved in redox regulation of the cell and protects radical-sensitive enzymes from oxidative damage by a radical-generating system. PRDX3 is required for MYC-mediated proliferation, transformation, and apoptosis after glucose withdrawal and essential for maintaining mitochondrial mass and membrane potential in transformed rat and human cells (PMID:12011429). PRDX3 acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.

Publikationen

Species	Application	Title
mouse	WB	Redox Biol; PMID: 32604037; Authors - Anindya Roy Chowdhury
human	WB,IHC	Redox Biol; LRPPRC regulates redox homeostasis via the circANKHD1/FOXM1 axis to enhance bladder urothelial carcinoma tumorigenesis.; Authors - Wen-Su Wei
human	WB	Redox Biol; Icariin alleviates uveitis by targeting peroxiredoxin 3 to modulate retinal microglia M1/M2 phenotypic polarization.; Authors - Guoqing Wang ; KD Validated
rat	IF	Oxid Med Cell Longev; Paeoniflorin Upregulates Mitochondrial Thioredoxin of Schwann Cells to Improve Diabetic Peripheral Neuropathy Indicated by 4D Label-Free Quantitative Proteomics.; Authors - Xinwei Yang
human	WB	Mol Cell Proteomics; Proteomics characterization of the cytotoxicity mechanism of ganoderic acid D and computer-automated estimation of the possible drug target network.; Authors - Yue Qing-Xi QX
		J Clin Endocrinol Metab; Thioredoxin reductase 2 (TXNRD2) mutation associated with familial glucocorticoid deficiency (FGD).; Authors - Rathi Prasad

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Guillermo (Verified Customer) (09-03-2022)

I trust in Proteintech, my favourite antibody company. 5/5 product.

• Applications: Western Blot