PRPF8 Polyklonaler Antikörper

PRPF8 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 11171-1-AP

Synonyme

PRP8 homolog, PRP8, Pre-mRNA-processing-splicing factor 8, p220, HPRP8

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 11171-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11171-1-AP (PRPF8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 11171-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11171-1-AP (PRPF8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11171-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11171-1-AP (PRPF8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11171-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11171-1-AP (PRPF8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11171-1-AP

  IP result of anti-PRPF8 (IP:11171-1-AP, 4ug; Detection:11171-1-AP 1:500) with HeLa cells lysate 2000ug.

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  IHC staining of mouse brain using 11171-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11171-1-AP (PRPF8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11171-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11171-1-AP (PRPF8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 11171-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11171-1-AP (PRPF8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, humanes Plazenta-Gewebe, K-562-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Produktinformation

11171-1-AP bindet in WB, IHC, IP, RIP, ELISA PRPF8 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRPF8 fusion protein Ag1652
• Vollständiger Name: PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 274 kDa
• Beobachtetes Molekulargewicht: 220 kDa
• GenBank-Zugangsnummer: BC064370
• Gene symbol: PRPF8
• Gene ID (NCBI): 10594
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRPF8, a 220kd protein,is a component of mammalian spliceosomes, large multiprotein complexes involved in the removal of introns from mRNA precursor. It has a central structural role in the architecture of the U5 small nuclear ribonucleoprotein (snRNP) particle.

Publikationen

Species	Application	Title
human	WB	Mol Cell; Mechanism of exon ligation by human spliceosome.; Authors - Xiechao Zhan
human	WB	Genes Dev; An integrative analysis of colon cancer identifies an essential function for PRPF6 in tumor growth.; Authors - Adam S Adler
human	WB	Nucleic Acids Res; Sequence-dependent recruitment of SRSF1 and SRSF7 to intronless lncRNA NKILA promotes nuclear export via the TREX/TAP pathway.; Authors - Misbah Khan
human	WB	Nucleic Acids Res; XAB2 depletion induces intron retention in POLR2A to impair global transcription and promote cellular senescence.; Authors - Shuai Hou
human	WB	RNA; Rearrangements within human spliceosomes captured after exon ligation.; Authors - Ilagan Janine O JO
human	IP	J Biol Chem; Identification of a 35S U4/U6.U5 Tri-snRNP Complex Intermediate in Spliceosome Assembly.; Authors - Zhe Chen