PACT Polyklonaler Antikörper

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• KD/KO Validated

PACT Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 10771-1-AP

Synonyme

DYT16, HSD 14, HSD14, PACT, PKR associated protein X, PKR associating protein X, PRKRA, RAX

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Galerie der Validierungsdaten

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  WB analysis using 10771-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10771-1-AP (PACT antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10771-1-AP

  WB result of PACT antibody (10771-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PACT transfected MCF-7 cells.

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  WB analysis of HeLa using 10771-1-AP

  WB result of PACT antibody (10771-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PACT transfected HeLa cells.

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  IHC staining of mouse testis using 10771-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10771-1-AP (PACT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 10771-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10771-1-AP (PACT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, C2C12-Zellen, MCF-7-Zellen, PC-12-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

10771-1-AP bindet in WB, IHC, IF, CoIP, ELISA PACT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PACT fusion protein Ag1214
• Vollständiger Name: protein kinase, interferon-inducible double stranded RNA dependent activator
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 31-34 kDa
• GenBank-Zugangsnummer: BC009470
• Gene symbol: PRKRA
• Gene ID (NCBI): 8575
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRKRA (PKR-associated protein X) is also named as PACT, RAX, HSD-14 and HSD14. PRKRA was significantly overexpressed in PC tissues and promoted the proliferation, migration, and chemoresistance of PC cells (PMID: 37484321). PRKRA activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. PRKRA has been shown to interact with DICER1. DICER1 and PRKRA expression in normal colon was limited to mucosal epithelium and showed similar cytoplasmic expression (PMID: 37484321).

Publikationen

Species	Application	Title
human	WB	Cell Rep; LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation; Authors - Nasha Zhang
rat	IF	EMBO Rep; The dynamic recruitment of TRBP to neuronal membranes mediates dendritogenesis during development.; Authors - Anna Antoniou
human	WB	Nucleic Acids Res; Hepato-specific microRNA-122 facilitates accumulation of newly synthesized miRNA through regulating PRKRA.; Authors - Li Shuai S
human	WB	PLoS Pathog; KSHV inhibits stress granule formation by viral ORF57 blocking PKR activation.; Authors - Nishi R Sharma
human	WB	J Proteome Res; Proteomic analysis of early HIV-1 nucleoprotein complexes.; Authors - Schweitzer Cameron J CJ
human,mouse	WB	J Biol Chem; PACT Establishes a Post-transcriptional Brake on Mitochondrial Biogenesis by Promoting the Maturation of miR-181c.; Authors - Asli E Dogan