PRKD3 Polyklonaler Antikörper

PRKD3 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 12785-1-AP

Synonyme

EPK2, PKC NU, PKD3, PRKCN, PRKD3, Protein kinase C nu type, protein kinase D3, Protein kinase EPK2

---

Galerie der Validierungsdaten

• 

  WB analysis using 12785-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12785-1-AP (PRKD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12785-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12785-1-AP (PRKD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DU 145 using 12785-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 12785-1-AP (PRKD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 12785-1-AP

  IP result of anti-PRKD3 (IP:12785-1-AP, 4ug; Detection:12785-1-AP 1:300) with HEK-293 cells lysate 2800ug.

• 

  IHC staining of human breast cancer using 12785-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12785-1-AP (PRKD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12785-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12785-1-AP (PRKD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12785-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12785-1-AP (PRKD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12785-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12785-1-AP (PRKD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DU 145-Zellen, C2C12-Zellen, HeLa-Zellen, NIH/3T3-Zellen, Raji-Zellen, Ramos-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

12785-1-AP bindet in WB, IP, IF, IHC, ELISA PRKD3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRKD3 fusion protein Ag3499
• Vollständiger Name: protein kinase D3
• Berechnetes Molekulargewicht: 611aa,68 kDa; 100 kDa; 890aa
• Beobachtetes Molekulargewicht: 68 kDa, 100 kDa
• GenBank-Zugangsnummer: BC030706
• Gene symbol: PRKD3
• Gene ID (NCBI): 23683
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IF,IP	Exp Mol Med; Clusterin negatively modulates mechanical stress-mediated ligamentum flavum hypertrophy through TGF-β1 signaling; Authors - Chunlei Liu
human	WB	NAR Cancer; Estrogen receptor alpha mutations regulate gene expression and cell growth in breast cancer through microRNAs; Authors - Spencer Arnesen