pan-PKC Polyklonaler Antikörper

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• KD/KO Validated

pan-PKC Polyklonal Antikörper für WB, IHC, FC (Intra), IP, ELISA, IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte, Zebrafisch

Anwendung

WB, IHC, FC (Intra), IP, ELISA, IF

Konjugation

Unkonjugiert

Publikationen

22

Rezensionen (2)

Kat-Nr. : 12919-1-AP

Synonyme

PKC beta, PRKCB, PKC-beta, PKC-B, PKCB

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Galerie der Validierungsdaten

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  WB analysis using 12919-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12919-1-AP (PKC Beta antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12919-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12919-1-AP (PKC beta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 12919-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12919-1-AP (PKC beta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12919-1-AP

  IP result of anti-pan-PKC (IP:12919-1-AP, 4ug; Detection:12919-1-AP 1:2000) with mouse brain tissue lysate 2100 ug.

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  IHC staining of human breast cancer using 12919-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12919-1-AP (PKC beta antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 12919-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12919-1-AP (PKC beta antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of zebrafish retina using 12919-1-AP

  Immunofluorescence analysis of bipolar cells labelled with pan-PKC polyclonal antibody (12919-1-AP, green) on tissue sections of the zebrafish retina at 5 days post fertilisation. Dilution 1:300. Tissue fixed overnight in 4% PFA. Müller glia, red; DAPI, blue; HuC/D, magenta. Data generated by Natalia Jaroszynska in Professor Ryan MacDonald's lab, University College London, UK.

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  FC experiment of K-562 using 12919-1-AP

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human PKC Beta (12919-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, Maushirngewebe, Maus-Thymusgewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: zebrafish retina
• Erfolgreiche Detektion in FC (Intra): K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:150-1:600
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 18 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

12919-1-AP bindet in WB, IHC, FC (Intra), IP, ELISA, IF pan-PKC und zeigt Reaktivität mit human, Maus, Ratten, Zebrafisch

• Getestete Reaktivität: human, Maus, Ratte, Zebrafisch
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: pan-PKC fusion protein Ag3591
• Vollständiger Name: protein kinase C, beta
• Berechnetes Molekulargewicht: 673 aa, 77 kDa
• Beobachtetes Molekulargewicht: 77 kDa
• GenBank-Zugangsnummer: BC036472
• Gene symbol: PRKCB
• Gene ID (NCBI): 5579
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. Protein kinase Cs (PKCs) are signaling molecules that play key roles in many cellular processes, including secretion, gene expression, proliferation, and muscle contraction. The PKC family is broadly divided into three subgroups: classical, novel, and atypical PKCs. These subgroups differ in both protein sequences and mechanistic requirements for catalytic activity. PKC beta is one of the PKC family members. PKC beta has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. This antibody can recognize PKC beta, PKC alpha, PKC gamma, PKC epsilon and PKC delta.

Publikationen

Species	Application	Title
human	IHC	J Hematol Oncol; Integrated proteogenomic characterization of urothelial carcinoma of the bladder.; Authors - Ning Xu
rat	WB	Am J Respir Cell Mol Biol; Intermittent Hypoxia Augments Pulmonary Vasoconstrictor Reactivity through PKCβ/Mitochondrial Oxidant Signaling.; Authors - Jessica B Snow ; KD Validated
human	WB	J Cell Mol Med; Brucine suppresses colon cancer cells growth via mediating KDR signalling pathway.; Authors - Luo Wenjuan W
human	WB	Oxid Med Cell Longev; p66Shc Mediates Mitochondrial Dysfunction Dependent on PKC Activation in Airway Epithelial Cells Induced by Cigarette Smoke.; Authors - Ming Zhang
human	WB	J Cell Mol Med; Prostaglandin E3 attenuates macrophage-associated inflammation and prostate tumour growth by modulating polarization.; Authors - Jing Cui
human	WB	Reprod Biol Endocrinol; In vitro fertilization with frozen embryo transfer increased histamine-mediated contractile sensitivity via PKCβ in human umbilical vein; Authors - Jiaqi Tang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ashish (Verified Customer) (05-21-2021)

This antibody gave very clear staining results in lung tissue. Very nice antibody to work.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: Lung

pan-PKC Antibody Immunohistochemistry validation (1:300 dilution) in Lung (Cat no:12919-1-AP)

FH

Amy (Verified Customer) (09-06-2019)

This antibody dilution gave very clear protein bands in all murine colon samples used to date. Following ECL addition, the imager took ~240 seconds to detect PKCbeta (automatic exposure). Very easy antibody to work with

• Applications: Western Blot,
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: Murine Colon Samples