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  • KD/KO Validated

AMPK Gamma 1 Polyklonaler Antikörper

AMPK Gamma 1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10290-1-AP

Synonyme

PRKAG1, AMPKG, AMPK subunit gamma-1, AMPK subunit gamma 1, AMPK gamma1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen, K-562-Zellen
Erfolgreiche IPK-562-Zellen
Erfolgreiche Detektion in IHChumanes Hodengewebe, humanes Skelettmuskelgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:4000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:20-1:200
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

KD/KOSee 2 publications below
WBSee 8 publications below

Produktinformation

10290-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA AMPK Gamma 1 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen AMPK Gamma 1 fusion protein Ag0302
Vollständiger Name protein kinase, AMP-activated, gamma 1 non-catalytic subunit
Berechnetes Molekulargewicht 38 kDa
Beobachtetes Molekulargewicht 35-38 kDa
GenBank-ZugangsnummerBC000358
Gene symbol AMPK Gamma 1
Gene ID (NCBI) 5571
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein kinase, AMP-activated, gamma 1 non-catalytic subunit (PRKAG1, synonyms: AMPKG, MGC8666) is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an catalytic subunit, and non-catalytic and subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and iAMP-activated protein kinase (AMPK) is a highly conserved heterotrimeric serine/threonine kinase widely characterised as a sensor of cellular energetic stress. AMPK is a heterotrimeric complex consisting of a catalytic α-subunit and two regulatory subunits (β and γ). AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. AMPK gamma 1 is one of the gamma regulatory subunits of AMPK.

Protokolle

Produktspezifische Protokolle
WB protocol for AMPK Gamma 1 antibody 10290-1-APProtokoll herunterladen
IHC protocol for AMPK Gamma 1 antibody 10290-1-APProtokoll herunterladen
IF protocol for AMPK Gamma 1 antibody 10290-1-APProtokoll herunterladen
IP protocol for AMPK Gamma 1 antibody 10290-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Cell Metab

CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress.

Authors - Qidong Li
  • KD Validated
mouseWB

JHEP Rep

Human umbilical cord mesenchymal stem cell-derived exosomes ameliorate liver steatosis by promoting fatty acid oxidation and reducing fatty acid synthesis

Authors - Fuji Yang
humanWB

J Med Chem

Identification of Pyruvate Carboxylase as the Cellular Target of Natural Bibenzyls with Potent Anticancer Activity against Hepatocellular Carcinoma via Metabolic Reprogramming.

Authors - Yuwen Sheng
humanWB

Protein Cell

AMPK interacts with DSCAM and plays an important role in netrin-1 induced neurite outgrowth.

Authors - Zhu Kun K
humanWB

J Exp Clin Cancer Res

METTL16 promotes glycolytic metabolism reprogramming and colorectal cancer progression

Authors - Wei Wei
  • KD Validated
humanWB

Cell Death Dis

WW domain-binding protein 2 overexpression prevents diet-induced liver steatosis and insulin resistance through AMPKβ1.

Authors - Zhe Zheng